Functional architecture of the cell nucleus: towards comprehensive toponome reference maps of apoptosis

Biochimica Et Biophysica Acta
Walter SchubertReyk Hillert

Abstract

We have recently described the MELC/TIS fluorescence robot technology that is capable of colocalizing at least a hundred different molecular cell components in one cell. The technology reveals new hierarchical properties of protein network organisation, referred to as the toponome, in which topologically confined protein clusters are interlocked within the structural framework of the cell. In this study we have applied MELC/TIS to construct a three-dimensional toponome map of the cell nucleus of a single human hepatocyte undergoing apoptosis. The map reveals six different spatially separated toponome domains in the nuclear interior of one apoptotic cell. In the drive to decipher the apoptosis-specific molecular network on the single cell level, the present toponome map is a first milestone towards the construction of much larger maps addressing hundreds of molecular cell components across the stages of apoptosis.

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Citations

Oct 29, 2011·IEEE Transactions on Visualization and Computer Graphics·Steffen OeltzeWalter Schubert
May 18, 2016·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Hernán E GreccoEli Zamir
Apr 15, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Walter Schubert

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