Functional assay, expression of growth factors and proteins modulating bone-arrangement in human osteoblasts seeded on an anorganic bovine bone biomaterial

European Cells & Materials
O TrubianiA Piattelli

Abstract

The basic aspects of bone tissue engineering include chemical composition and geometry of the scaffold design, because it is very important to improve not only cell attachment and growth but especially osteodifferentiation, bone tissue formation, and vascularization. Geistlich Bio-Oss (GBO) is a xenograft consisting of deproteinized, sterilized bovine bone, chemically and physically identical to the mineral phase of human bone. In this study, we investigated the growth behaviour and the ability to form focal adhesions on the substrate, using vinculin, a cytoskeletal protein, as a marker. Moreover, the expression of bone specific proteins and growth factors such as type I collagen, osteopontin, bone sialoprotein, bone morphogenetic protein-2 (BMP-2), BMP-7 and de novo synthesis of osteocalcin in normal human osteoblasts (NHOst) seeded on xenogenic GBO were evaluated. Our observations suggest that after four weeks of culture in differentiation medium, the NHOst showed a high affinity for the three dimensional biomaterial; in fact, cellular proliferation, migration and colonization were clearly evident. The osteogenic differentiation process, as demonstrated by morphological, histochemical, energy dispersive X-ray microanalysis an...Continue Reading

Citations

Oct 1, 2011·International Journal of Immunopathology and Pharmacology·E CenniN Baldini
Dec 8, 2016·Scientific Reports·Thangavelu Soundara RajanOriana Trubiani
Jun 9, 2017·Journal of Tissue Engineering and Regenerative Medicine·R SinibaldiS Della Penna
Nov 21, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Caroline SzpalskiStephen M Warren
Nov 19, 2011·Clinical Oral Implants Research·Živko MladenovićMaria Ransjö
Sep 26, 2012·Molecular Medicine Reports·Shao-Hui Yuan, Zhenggang Bi
Dec 20, 2018·International Journal of Molecular Sciences·Jacopo PizzicannellaFrancesca Diomede
Oct 28, 2011·Tissue Engineering. Part B, Reviews·Caroline SzpalskiStephen M Warren
Sep 3, 2013·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Stefano CecconiAntonio Gigante

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