Functional assessment of cell entry and receptor usage for SARS-CoV-2 and other lineage B betacoronaviruses

Nature Microbiology
Michael LetkoVincent Munster

Abstract

Over the past 20 years, several coronaviruses have crossed the species barrier into humans, causing outbreaks of severe, and often fatal, respiratory illness. Since SARS-CoV was first identified in animal markets, global viromics projects have discovered thousands of coronavirus sequences in diverse animals and geographic regions. Unfortunately, there are few tools available to functionally test these viruses for their ability to infect humans, which has severely hampered efforts to predict the next zoonotic viral outbreak. Here, we developed an approach to rapidly screen lineage B betacoronaviruses, such as SARS-CoV and the recent SARS-CoV-2, for receptor usage and their ability to infect cell types from different species. We show that host protease processing during viral entry is a significant barrier for several lineage B viruses and that bypassing this barrier allows several lineage B viruses to enter human cells through an unknown receptor. We also demonstrate how different lineage B viruses can recombine to gain entry into human cells, and confirm that human ACE2 is the receptor for the recently emerging SARS-CoV-2.

References

Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·A TakadaY Kawaoka
Aug 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Shutoku MatsuyamaFumihiro Taguchi
Sep 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Susanna K P LauKwok-Yung Yuen
Oct 1, 2005·Science·Wendong LiLin-Fa Wang
Feb 21, 2006·Emerging Infectious Diseases·Ming WangJianguo Xu
Nov 28, 2008·Proceedings of the National Academy of Sciences of the United States of America·Michelle M BeckerMark R Denison
Mar 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sandrine BelouzardGary R Whittaker
Oct 19, 2011·The Journal of Infectious Diseases·Andrea MarziHeinz Feldmann
Aug 13, 2014·Proceedings of the National Academy of Sciences of the United States of America·Yang YangFang Li
Nov 25, 2014·Emerging Infectious Diseases·Danielle R AdneyVincent J Munster
Dec 3, 2014·Virus Research·Jean Kaoru Millet, Gary R Whittaker
Nov 10, 2015·Nature Medicine·Vineet D MenacheryRalph S Baric
Sep 1, 2016·Annual Review of Virology·Fang Li
Aug 16, 2018·Cell Reports·Michael LetkoVincent Munster
Nov 5, 2019·The Journal of Infectious Diseases·Stephanie N SeifertVincent J Munster
Dec 6, 2019·Journal of Virology·Vineet D MenacheryRalph S Baric

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Citations

Apr 8, 2020·Circulation Research·Akbarshakh Akhmerov, Eduardo Marban
Apr 20, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tiantian YanGuoan Lin
Apr 1, 2020·Nature·Jian ShangFang Li
Apr 4, 2020·Journal of Medical Virology·Yifei ChenZhenshun Cheng
Apr 16, 2020·Journal of Medical Virology·Adam Brufsky
Apr 3, 2020·Journal of Medical Virology·Qing YeTing Zhang
Apr 25, 2020·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Xiaoxia LuGary Wing-Kin Wong
May 7, 2020·Journal of Human Genetics·Kazuma KiyotaniYusuke Nakamura
May 6, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rami A BalloutAlan T Remaley
May 23, 2020·PLoS Pathogens·Daolin TangRui Kang
May 24, 2020·International Journal of Molecular Sciences·Lucia LonghitanoArcangelo Liso
May 28, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Elisa BarileMaurizio Pellecchia
May 20, 2020·The FEBS Journal·Oleg O Glebov
May 29, 2020·Journal of Medical Virology·Nevio Cimolai
May 20, 2020·The Pediatric Infectious Disease Journal·Ulrich Heininger
May 28, 2020·Clinical Chemistry and Laboratory Medicine : CCLM·Mary Kathryn BohnKhosrow Adeli
May 22, 2020·Journal of Medical Virology·Dandan Tian, Qing Ye
May 27, 2020·Andrology·Donatella PaoliFrancesco Lombardo
May 25, 2020·Dermatologic Therapy·Valeria GaspariFederico Bardazzi
Jun 23, 2020·Future Medicinal Chemistry·Siddhi JoshiMariam S Degani
Jun 24, 2020·Khirurgiia·Sh V TimerbulatovV M Timerbulatov
Jun 12, 2020·Autophagy·Srinivasa Reddy BonamDaniel J Klionsky
Jun 26, 2020·Current Protocols in Microbiology·Fatima AmanatFlorian Krammer
Apr 14, 2020·Journal of Cardiovascular Pharmacology·Dave L DixonAntonio Abbate
Jun 5, 2020·British Journal of Pharmacology·Chiara ColarussoRosalinda Sorrentino
Jun 13, 2020·Expert Review of Respiratory Medicine·Arafat HussainAnant Mohan
Jul 3, 2020·Phytotherapy Research : PTR·Helda TutunchiMohammad Javad Hosseinzadeh-Attar
May 10, 2020·Dermatologic Therapy·Nawaf Almutairi, Robert A Schwartz
Jul 9, 2020·Experimental Biology and Medicine·Henok Kessete Afewerky
Jul 10, 2020·Critical Reviews in Clinical Laboratory Sciences·Marco CiottiSergio Bernardini
Jul 8, 2020·Pathogens·Ahmed S Abdel-Moneim, Elsayed M Abdelwhab
Jul 16, 2020·Journal of Cardiac Surgery·Surendra PatelAlok Kumar Sharma
May 21, 2020·American Journal of Respiratory and Critical Care Medicine·Haijun ZhangRonald G Crystal
May 3, 2020·British Journal of Pharmacology·Steve P H AlexanderMichael Spedding
May 30, 2020·Ocular Immunology and Inflammation·Maria CasagrandeMaximilian Schultheiss

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Methods Mentioned

BETA
flow cytometry
PCR

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