Functional asymmetry of photosystem II D1 and D2 peripheral chlorophyll mutants of Chlamydomonas reinhardtii

Proceedings of the National Academy of Sciences of the United States of America
Jun WangR T Sayre

Abstract

The peripheral accessory chlorophylls (Chls) of the photosystem II (PSII) reaction center (RC) are coordinated by a pair of symmetry-related histidine residues (D1-H118 and D2-H117). These Chls participate in energy transfer from the proximal antennae complexes (CP43 and CP47) to the RC core chromophores. In addition, one or both of the peripheral Chls are redox-active and participate in a low-quantum-yield electron transfer cycle around PSII. We demonstrate that conservative mutations of the D2-H117 residue result in decreased Chl fluorescence quenching efficiency attributed to reduced accumulation of the peripheral accessory Chl cation, Chl(Z)(+). In contrast, identical symmetry-related mutations at residue D1-H118 had no effect on Chl fluorescence yield or quenching kinetics. Mutagenesis of the D2-H117 residue also altered the line width of the Chl(Z)(+) EPR signal, but the line shape of the D1-H118Q mutant remained unchanged. The D1-H118 and D2-H117 mutations also altered energy transfer properties in PSII RCs. Unlike wild type or the D1-H118Q mutant, D2-H117N RCs exhibited a reduced CD doublet in the red region of Chl absorbance band, indicative of reduced energetic coupling between P680 and the peripheral accessory Chl. I...Continue Reading

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Citations

Sep 7, 2005·Photosynthesis Research·Jun Minagawa, Yuichiro Takahashi
Nov 20, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Alison Telfer
Apr 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·A R HolzwarthM Rögner
Nov 21, 2015·Journal of the American Chemical Society·Daniele NarziLeonardo Guidoni
May 3, 2011·Journal of Photochemistry and Photobiology. B, Biology·Harvey J M Hou, David Mauzerall
Mar 16, 2011·Journal of Photochemistry and Photobiology. B, Biology·Roberta Croce, Herbert van Amerongen
Apr 3, 2015·Photosynthesis Research·Jinhai ChenRyszard Jankowiak
Nov 25, 2006·Biochimica Et Biophysica Acta·Hiroshi IshikitaErnst-Walter Knapp
Aug 26, 2011·Biochimica Et Biophysica Acta·Katherine E Shinopoulos, Gary W Brudvig
Feb 19, 2020·Protein Science : a Publication of the Protein Society·Frank Müh, Athina Zouni
Jun 28, 2018·Photosynthesis Research·Lucyna M WlodarczykIvo H M Stokkum
Apr 27, 2010·Biochemistry·Elisabet RomeroRienk van Grondelle
Apr 3, 2012·The Journal of Physical Chemistry. B·Khem AcharyaRyszard Jankowiak

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