Functional calcium-sensing receptors in rat fibroblasts are required for activation of SRC kinase and mitogen-activated protein kinase in response to extracellular calcium.

The Journal of Biological Chemistry
Scott E McNeilKarin D Rodland

Abstract

Changes in the concentration of extracellular calcium can affect the balance between proliferation and differentiation in several cell types, including keratinocytes, breast epithelial cells, and fibroblasts. This report demonstrates that elevation of extracellular calcium stimulates proliferation-associated signaling pathways in rat fibroblasts and implicates calcium-sensing receptors (CaR) as mediators of this response. Rat-1 fibroblasts express CaR mRNA and protein and respond to known agonists of the CaR with increased IP3 production and release of intracellular calcium. Agonists of the CaR can stimulate increased c-SRC kinase activity and increased extracellular signal-regulated kinase 1/mitogen-activated protein kinase activity. Both of the increases in SRC activity and mitogen-activated protein kinase activation are blocked in the presence of a nonfunctional mutant of the CaR, R796W. Proliferation of wild-type Rat-1 cells is sensitive to changes in extracellular calcium, but expression of the nonfunctional CaR mutant or inhibition of the calcium-dependent increase in SRC kinase activity block the proliferative response to calcium. These results provide evidence of a novel signal transduction pathway modulating the respon...Continue Reading

References

Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·Y ZhaoJ Krueger
Dec 1, 1989·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·B L Black, J E Smith
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·M RuatS H Snyder
May 26, 1995·The Journal of Biological Chemistry·J E GarrettF Fuller
Jul 27, 1995·The New England Journal of Medicine·E M BrownS C Hebert
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·D RiccardiS C Hebert
Dec 1, 1993·Molecular and Cellular Biology·Y ZhaoH Hanafusa
Mar 15, 1996·The Journal of Biological Chemistry·M RuatS H Snyder

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Citations

Jul 5, 2008·Journal of Bone and Mineral Metabolism·Toru Yamaguchi
Jul 19, 2005·Journal of Mammary Gland Biology and Neoplasia·Joshua N VanHouten
Jun 18, 2004·Cell Calcium·Karin D Rodland
Jun 18, 2004·Cell Calcium·Donald T Ward
Oct 26, 2000·Trends in Pharmacological Sciences·A D ConigraveE M Brown
Mar 20, 2003·Molecular and Cellular Endocrinology·Susan A HobsonKarin D Rodland
Apr 15, 2008·Trends in Endocrinology and Metabolism : TEM·N ChattopadhyayE M Brown
Aug 15, 2000·The International Journal of Biochemistry & Cell Biology·N Chattopadhyay
Feb 15, 2011·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Keiichi HizakiYasuhisa Shinomura
Oct 5, 2011·The Journal of Biological Chemistry·Sujeenthar TharmalingamDavid R Hampson
Apr 11, 2000·Current Opinion in Nephrology and Hypertension·J W Coburn, H M Maung
Mar 24, 2010·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Roger J NarayanJeffrey W Elam
Jun 17, 1999·Alcoholism, Clinical and Experimental Research·K S CrillyZ Kiss
Jul 7, 2009·Annual Review of Physiology·John P Geibel, Steven C Hebert
Aug 8, 2008·American Journal of Physiology. Renal Physiology·Huda Ismail AbdullahNicholas R Ferreri
Jun 26, 2012·Circulation Research·Aya YamamuraJason X-J Yuan
May 3, 2000·The Journal of Clinical Investigation·I T OlszakD T Scadden
Oct 23, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T YamaguchiE M Brown
Aug 24, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M PiL d Quarles
Mar 8, 2006·Clinical Chemistry and Laboratory Medicine : CCLM·Indra Ramasamy
Jun 6, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Teresa De SantisMaria Elena Dell'Aquila
Nov 4, 2000·Biochemical and Biophysical Research Communications·A Farzaneh-FarC M Shanahan
Jun 1, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Li-Juan XiaoYi-Xun Liu
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·M HershfinkelI Sekler
Nov 18, 2008·Endocrine·Bandana ChakravartiNaibedya Chattopadhyay
Jan 29, 2002·Free Radical Research·F Dal-PizzolJ C Moreira
Mar 12, 2004·Expert Opinion on Investigational Drugs·Alberto Falchetti
Sep 14, 2007·Archives of Physiology and Biochemistry·Peter M JonesShanta J Persaud
Sep 25, 2008·Kidney International·Min PiL Darryl Quarles
Aug 10, 2000·Neoplasia : an International Journal for Oncology Research·A O HoffR F Gagel
Dec 29, 2015·Seminars in Cell & Developmental Biology·Jennifer L OwenMansour Mohamadzadeh
Sep 1, 1999·The Journal of Investigative Dermatology·C L TuD D Bikle
May 30, 2008·General and Comparative Endocrinology·Maarten J J van den HurkBruce G Jenks

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