Functional cardiac Na+ channels are expressed in human melanoma cells

Oncology Letters
An XieYinsheng Wan

Abstract

Resting membrane potential (RMP) and intracellular Ca2+ concentration [(Ca2+)i] are involved in tumorigenesis and metastasis. The present study investigated whether functional cardiac Na+ channels are expressed in human melanoma cells (WM 266-4) and its nonmalignant human melanocytes (HMC), as well as whether they participate in RMP maintenance and Ca2+ homeostasis. Confocal microscopy and western blot analysis were used to detect Na+ channels. The patch-clamp technique was employed to record Na+ currents and action potentials. Cytoplasmic Ca2+ was measured by loading Fluo-4. Cardiac (Nav1.5) Na+ channels were expressed in HMCs and WM 266-4 cells. Tetrodotoxin (TTX) dose-dependently blocked Na+ currents in WM 266-4 while HMCs had no Na+ currents. Ultraviolet light induced similar action potentials in HMCs and WM 266-4 cells, which were abolished by transient receptor potential A1 channel-specific blocker, HC-030031. Compared with HMCs, RMP was substantially depolarized in WM 266-4. TTX hyperpolarized RMP in WM 266-4 cells at a concentration of 30 µM, which facilitated Ca2+ influx. Compared with HMCs, (Ca2+)i was significantly higher in WM 266-4 cells and was elevated by 30 µM TTX. Collectively, Cardiac Na+ channels depolarize R...Continue Reading

References

Jan 1, 1992·The Journal of Physiology·B Nilius, W Wohlrab
Dec 1, 1993·The American Journal of Physiology·B NiliusG Droogmans
May 1, 1996·The Journal of Membrane Biology·A Lepple-WienhuesM Wiederholt
Jan 1, 1997·The Journal of Membrane Biology·D H AllenM D Cahalan
Jan 13, 2009·The Journal of Biological Chemistry·Michael D CarrithersStephen G Waxman
Sep 12, 2009·Circulation Research·Man LiuSamuel C Dudley
Jan 21, 2012·Pigment Cell & Melanoma Research·A DasC Cantí
Mar 14, 2012·Marine Drugs·Francisco Rafael NietoCruz Miguel Cendán
Jan 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Nicholas W BellonoElena Oancea
Jun 15, 2013·Channels·Nicholas W Bellono, Elena Oancea
Jan 29, 2014·The Journal of General Physiology·Nicholas W BellonoElena Oancea
Jan 18, 2015·International Immunopharmacology·Hao YangWei-Dong Sun
Nov 3, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Qin XuRichard D Veenstra
May 12, 2017·Scientific Reports·Lyndsey L AndersonAlfred L George

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Software Mentioned

SigmaPlot
MetaMorph
Systat

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.