Functional changes in murine mammary cancer cells elicited by CoCl2-induced hypoxia

Nitric Oxide : Biology and Chemistry
Ximena BorensteinMaría A Jasnis

Abstract

Low O(2) levels in solid tumors are associated with increase in hypoxia-inducible factor 1alpha (HIF-1alpha). The present study examines functional changes involved in adaptation to hypoxia of the LMM3 mammary tumor cell line, using CoCl(2) as hypoxic mimetic. Our results showed that LMM3 cells were not only tolerant to 150 microM CoCl(2) but they can overgrowth in vitro respect to untreated cells. Hypoxia inhibited cell invasion, migration, MMP-9 activity and NO levels. Macrophage cytotoxicity augmented under hypoxia but was blunted by conditioned media from tumor cells. In vivo tumorigenicity of CoCl(2)-treated cells was greater than controls. Our results show stabilization of HIF-1alpha in LMM3 cells under CoCl(2) and functional changes associated with enhanced cell survival and growth but not with tumor dissemination.

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Citations

Sep 21, 2011·PloS One·Julie E GleasonValeria C Culotta
Jul 27, 2011·Environmental Toxicology and Pharmacology·Jong Woo ChungHong-Seob So
Aug 9, 2011·International Journal of Cancer. Journal International Du Cancer·Jeon Hwang-BoIn Sik Chung
Oct 18, 2016·Biochimie·María Noel GalardoSilvina Beatriz Meroni
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Jan 28, 2015·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Zhichao FuFengmei Wang
Dec 6, 2017·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Z C SongR Shu

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