Functional characterisation of bioactive peptide derived from terrestrial snail Cryptozona bistrialis and its wound-healing property in normal and diabetic-induced Wistar albino rats.

International Wound Journal
Selvakumari UlagesanAmutha Kuppusamy

Abstract

A peptide might be an exciting biomaterial or template for the development of novel wound-healing agents. In this report, it was isolated from the terrestrial snail Cryptozona bistrialis by enzymatic digestion and was evaluated for its in vitro wound-healing activity in NIH/3T3 mouse fibroblasts cell line and in vivo wound-healing activity in normal and diabetic-induced Wistar albino rats. The C. bistrialis protein was digested by the papain enzyme, and 21.79 kDa peptide (Cb-peptide) was purified by reversed-phase high-performance liquid chromatography and identified by MALDI (matrix-assisted laser desorption/ionization)-TOF analysis. The isolated Cb-peptide was characterised by various analytical methods. The peptide demonstrated a capacity to prevent the development of pathogenic bacterial and fungal cultures and proved that it promotes significant wound-healing activity in the wound scratch assay method by rapid cell migration and closure of wound. Isolated Cb-peptide was lyophilised and formulated to ointment and analysed for in vivo wound-healing activity in normal and diabetic (alloxan monohydrate)-induced Wistar albino rats. Cb-peptide ointment-treated groups showed a greater degree of wound healing and early and complet...Continue Reading

References

Jan 1, 1989·Toxicon : Official Journal of the International Society on Toxinology·H KamiyaM Yamazaki
Feb 1, 1986·The Journal of Surgical Research·K P SmithR H Didlake
Aug 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R Raghow
Feb 28, 2001·Diabetes, Obesity & Metabolism·J N MansbridgeG K Naughton
Jul 20, 2001·Annals of the New York Academy of Sciences·R A Clark
Jun 6, 2003·Journal of Wound Care·M Flanagan
May 4, 2004·Journal of Ethnopharmacology·R BoominathanS K Ghoshal
Feb 1, 2005·The American Journal of Pathology·Sang Gyu ParkSunghoon Kim
Mar 21, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Yamile GonzálezMaria A Chávez
Jul 1, 1993·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·B BucaloV Falanga
Feb 6, 2007·Clinics in Dermatology·Nathan B MenkeRobert F Diegelmann
Feb 8, 2008·Journal of Ethnobiology and Ethnomedicine·Cassandra L QuaveBradley C Bennett
Jan 30, 2009·Natural Product Reports·John W BluntMichèle R Prinsep
Feb 1, 1984·International Journal of Cosmetic Science·M AdolpheJ Wepierre
Jan 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Piyush KoriaMartin L Yarmush
Feb 5, 2011·Journal of Ethnopharmacology·Jacqueline Chor Wing TamClara Bik San Lau
Dec 17, 2014·Frontiers in Immunology·Nan WangKe Zen
Dec 24, 2015·Advanced Materials·Steven A CastleberryPaula T Hammond

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Jan 12, 2021·Complementary Therapies in Clinical Practice·Saeed Ebrahimi FanaMahmood Maniati
Feb 5, 2021·International Journal of Biological Macromolecules·Yiming SongJike Lu

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