PMID: 9172389Jul 1, 1997Paper

Functional characterisation of Eskimo dog hemoglobin: I. Interaction of Cl- and 2,3-DPG and its importance to oxygen unloading at low temperature

Comparative Biochemistry and Physiology. Part A, Physiology
A BardgardO Brix

Abstract

The oxygen binding properties of hemoglobin and some hematological parameters in Eskimo dogs (belonging to Canis lupus familiaris) in Ilulissat/Jacobshavn, Greenland were analysed. The average [2,3-DPG] and [Hb] (n = 16) were 3.14 +/- 0.34 mmol l-1 blood and 9.53 +/- 0.65 g dl-1 (1.49 mmol l-1), respectively, giving a stoichiometric ratio of 2.11 mol 2,3-DPG/mol Hb. Oxygen binding analysis carried out on hemolysate in HEPES buffer at 20 and 37 degrees C revealed a high oxygen affinity (1.2 mmHg at pH 7.4, 20 degrees C) in the desalted condition, which decreased markedly in the presence of chloride and 2,3-DPG. A low apparent equilibrium constant for the binding of 2,3-DPG (1.0 x 10(-5) mol l-1) was found at pH 7.2 and 20 degrees C in the absence of chloride. Moreover, we show that chloride ions have an additive effect on oxygen affinity in the concentration range 10-300 mmol l-1 in the presence of 3 mmol l-1 2,3-DPG at low pH and temperature (pH < 7.4 and 20 degrees C). This feature may be of physiological importance to oxygen unloading under acidotic conditions when tissue temperature is low. Thermodynamic analysis reveal that in the presence of 3 mmol l-1 2,3-DPG and 100 mmol l-1 chloride, the Eskimo dog hemoglobin exhibits a...Continue Reading

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Citations

Jul 1, 1997·Comparative Biochemistry and Physiology. Part A, Physiology·A J Bårdgard, O Brix
Jan 26, 2005·Biochimie·Massimo PomponiKit M Kovacs
Nov 24, 2012·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Inge G RevsbechAngela Fago
Apr 5, 2000·The Journal of Biological Chemistry·R E WeberL Moens
Mar 23, 2017·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Inge G Revsbech, Angela Fago
Jan 17, 2012·The Journal of Experimental Biology·Anthony V SignoreKevin L Campbell

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