Functional characterisation of OsAMT1.1 overexpression lines of rice, Oryza sativa

Functional Plant Biology : FPB
Anshuman KumarAnthony D M Glass

Abstract

In rice (Oryza sativa L.) OsAMT1.1 is the most active and / or most N-responsive gene responsible for high-affinity NH4+ transport (HATS) activity. We measured 13NH4+ influx and plant biomass in transgenic overexpression lines and two wild type cultivars of rice, Jarrah and Taipei, with one or more copies of OsAMT1.1. 13NH4+ influx was higher for the overexpression lines of Jarrah line when grown at 10 µm external NH4+ concentration, but not for the overexpression lines of Taipei. For seedlings grown at 2 mm external NH4+ concentration Jarrah lines 77-1 and 75-4 showed an increased influx; however, two overexpression lines of Taipei showed reduced influx rates. The biomasses of the transgenic lines grown at low and high external NH4+ concentrations were either reduced or showed no statistically significant differences compared with wild type lines. While 13NH4+ influx into roots of Jarrah line 75-4 grown at 10 µm external NH4+ concentration was significantly higher than in wild type, measurements of 13NH efflux revealed no differences, and thus net uptake of NH4+ was higher in this overexpression line.

References

Oct 31, 1980·Biochemical and Biophysical Research Communications·G Hummelt, J Mora
Sep 4, 1999·The Plant Journal : for Cell and Molecular Biology·S R RawatA D Glass
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·D T BrittoH J Kronzucker
Sep 1, 1967·Plant Physiology·G S Puritch, A V Barker

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Citations

Oct 1, 2015·Functional Plant Biology : FPB·Trevor GarnettMamoru Okamoto
Nov 10, 2017·The New Phytologist·Mechthild Tegeder, Céline Masclaux-Daubresse
May 24, 2020·International Journal of Molecular Sciences·Dong-Li HaoYan-Hua Su
Jan 10, 2012·Annual Review of Plant Biology·Guohua XuAnthony J Miller
Dec 1, 2014·Plant Cell Reports·Shunying YangYanhua Su
Mar 31, 2016·Plant & Cell Physiology·Takatoshi Kiba, Anne Krapp
Oct 20, 2018·BMC Plant Biology·Yafei SunShubin Sun
May 1, 2020·Frontiers in Plant Science·Dujun WangXingming Lian
May 6, 2020·The Plant Journal : for Cell and Molecular Biology·Sichul LeeHong Gil Nam
Mar 19, 2021·Frontiers in Plant Science·Samantha Vivia TheMechthild Tegeder
Aug 25, 2010·The Journal of Physical Chemistry. B·Thomas P NygaardCarme Rovira

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Datasets Mentioned

BETA
AF289478
AF289479
AF289477

Methods Mentioned

BETA
transgenic
PCR

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