PMID: 8938653Nov 1, 1996Paper

Functional characterisation of P2 purinoceptors in PC12 cells by measurement of radiolabelled calcium influx

Naunyn-Schmiedeberg's Archives of Pharmacology
A D MichelP P Humphrey

Abstract

The aim of this study was to determine whether 45Ca2+ influx could be used as a quantitative measure of channel activation for functional characterisation of P2X purinoceptors in cell lines. In undifferentiated PC12 cells, grown in suspension, ATP (EC50 = 45 microM), ATP gamma S (EC50 = 50 microM) and 2-meSATP (EC50 = 81 microM) but not alpha beta meATP (1 mM) stimulated 45Ca2+ influx 2-5 fold. This effect did not appear to be due to activation of P2U or P2Y purinoceptors since 1 mM UTP, ADP or ADP beta S did not produce any significant effect. Similarly, the effects of ATP were not apparently mediated through activation of P2Z purinoceptors since dibenzylATP behaved as a weak (EC50 = 191 microM) partial agonist (Maximal effect 29.5% of ATP maximum) and there was no detectable ATP-stimulated ethidium bromide uptake in the PC12 cells. ATP-stimulated 45Ca2+ influx was not affected by nifedipine suggesting that it was not secondary to activation of L-type calcium channels and rather reflected influx through a P2X purinoceptor present in these cells. The ATP-stimulated 45Ca2+ influx could be reduced by monovalent cations, presumably as a result of direct competition for influx through the cation channel, with the following rank ord...Continue Reading

Citations

May 2, 2006·Pflügers Archiv : European journal of physiology·Joel R GeverAnthony P D W Ford
Jun 26, 2013·Purinergic Signalling·Geoffrey Burnstock, Francesco Di Virgilio
Mar 23, 2004·European Journal of Pharmacology·Paul M LundyThomas W Sawyer
Jan 14, 1999·Neuropharmacology·K J MillerP P Humphrey
Dec 24, 1998·British Journal of Pharmacology·A D MichelP P Humphrey
Jun 18, 2003·The Journal of General Physiology·Andrei SkorinkinRashid Giniatullin
Oct 14, 2004·European Journal of Biochemistry·J Russel Keath, Edward W Westhead
Nov 2, 2007·Clinical and Experimental Pharmacology & Physiology·Ji-Hu SunZheng-Hua Xiang
Jul 9, 2011·Pharmacological Reviews·Claudio CoddouStanko S Stojilkovic
Mar 23, 2000·Neuropharmacology·N D'AmbrosiC Volonté
Dec 22, 1999·The Journal of Physiology·M Omatsu-Kanbe, H Matsuura
Aug 5, 2008·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·R R ResendeH Ulrich
Mar 19, 2003·British Journal of Pharmacology·Vladimir S VorobjevOlga A Sergeeva
Apr 12, 2018·International Journal of Molecular Sciences·Milos B RokicClaudio Coddou
Sep 25, 2004·Journal of Neuroscience Research·Linda J VicianHarvey R Herschman
Sep 17, 2004·Journal of Neuroscience Research·Holger Peter Behrsing, P Richard Vulliet
Feb 16, 2017·MBio·Gisela von HovenMatthias Husmann
Jan 29, 1999·Physiological Reviews·B D SchultzR J Bridges
Feb 12, 2010·Journal of Medicinal Chemistry·Younis BaqiChrista E Müller
Jan 7, 2011·Journal of Medicinal Chemistry·Younis BaqiChrista E Müller
Jan 9, 2010·Cellular Signalling·Simona DanieleClaudia Martini

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