Functional characteristics of chemosensory proteins in the sawyer beetle Monochamus alternatus Hope

Bulletin of Entomological Research
S AliM-Q Wang

Abstract

The Japanese pine sawyer, Monochamus alternatus Hope (Coleoptera: Cerambycidae), is a major pest of pines and it is also the key vector of the exotic pinewood nematode in China. In the present study, we cloned, expressed, and purified a chemosensory protein (CSP) in M. alternatus. We surveyed its expression in various developmental stages of male and female adult tissues and determined its binding affinities for different pine volatiles using a competitive binding fluorescence assay. A CSP known as CSP5 in M. alternatus was obtained from an antennal cDNA library and expressed in Escherichia coli. Quantitative reverse transcription polymerase chain reaction results indicated that the CSP5 gene was mainly expressed in male and female antennae. Competitive binding assays were performed to test the binding affinity of recombinant CSP5 to 13 odour molecules of pine volatiles. The results showed that CSP5 showed very strong binding abilities to myrcene, (+)-β-pinene, and (-)-isolongifolene, whereas the volatiles 2-methoxy-4-vinylphenol, p-cymene, and (+)-limonene oxide have relatively weak binding affinity at pH 5.0. Three volatiles myrcene, (+)-β-pinene, and (-)-isolongifolene may play crucial roles in CSP5 binding with ligands but ...Continue Reading

References

Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R G VogtG D Prestwich
Jan 1, 1987·Annals of the New York Academy of Sciences·K E KaisslingE R Rumbo
Nov 10, 1994·Biochimica Et Biophysica Acta·H BreerJ Krieger
Sep 27, 2000·Proceedings of the National Academy of Sciences of the United States of America·F OduolK D Vernick
Oct 4, 2000·Chemical Senses·P Nagnan-Le MeillourR A Steinbrecht
Dec 21, 2000·Insect Molecular Biology·L M FieldL J Wadhams
Nov 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·R HorstK Wüthrich
Jun 11, 2002·Biochemical and Biophysical Research Communications·Liping BanPaolo Pelosi
Jun 18, 2002·The Journal of Biological Chemistry·Audrey LartigueChristian Cambillau
Apr 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Valerie CampanacciChristian Cambillau
May 8, 2004·Trends in Biochemical Sciences·Mariella TegoniChristian Cambillau
Jul 28, 2004·Journal of Chemical Ecology·Kevin W WannerErika Plettner
Feb 16, 2005·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Carol I MilesJ Alan A Renwick
Dec 19, 2006·Insect Biochemistry and Molecular Biology·Sylvain ForêtRyszard Maleszka
Feb 14, 2007·Insect Biochemistry and Molecular Biology·Da-Ping GongZhong-Huai Xiang
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Feb 28, 2009·PloS One·Christelle RobinetJianghua Sun
Apr 15, 2009·Insect Biochemistry and Molecular Biology·Richard G VogtJoseph C Dickens
Jun 2, 2009·Journal of Molecular Biology·Marion E PesentiChristian Cambillau
Jun 16, 2009·Insect Molecular Biology·D GonzálezR Renthal
Feb 21, 2013·PloS One·Praveen MamidalaOmprakash Mittapalli

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.