PMID: 3747521Aug 1, 1986Paper

Functional characteristics of nuclear 5 alpha-reductase from rat ventral prostate

Journal of Steroid Biochemistry
U Enderle-SchmittG Aumüller

Abstract

The subcellular distribution and functional characteristics of 5 alpha-reductase (3-oxo-5 alpha-steroid: NADP+ 4-ene-oxidoreductase, EC 1.3.1.22) from rat ventral prostate were studied and compared to the 5 alpha-reductase from female rat liver. Tissue fractionation retained main enzymic activity in the microsomal fraction of rat liver, while 5 alpha-reductase from rat prostate was localized in the nuclear membrane with a specific activity 160 times that of the initial homogenate. The purity of nuclear envelopes was checked by electron microscopy. Solubilization experiments indicated that the hepatic 5 alpha-reductase is attached to the endoplasmic reticulum as a peripheral protein, while the nuclear prostatic enzyme is an integral membrane protein. Incubation experiments with phospholipases suggested a decisive role of the surrounding phospholipids for the prostatic enzyme activity. To elucidate the characteristics of hydrogen transfer of the enzyme, the effect of flavins and different other cofactors on 5 alpha-reductase activity in isolated prostatic nuclei were studied. Our findings indicate that in rat ventral prostate the conversion of testosterone to 5 alpha-dihydrotestosterone proceeds by a direct hydrogen transfer from...Continue Reading

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Citations

Jan 1, 1989·Anatomy and Embryology·G Aumüller
Jan 1, 1990·Archives of Dermatological Research·S KurataS Takayasu
Sep 1, 1989·Journal of Steroid Biochemistry·U Enderle-SchmittG Aumüller
Apr 28, 1995·Molecular and Cellular Endocrinology·J G SavoryY A Lefebvre
Mar 1, 1991·Journal of Dermatological Science·S KurataS Takayasu
Oct 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·A B OrdmanH Nick
May 16, 1997·Molecular and Cellular Endocrinology·A PolettiL Martini
Apr 1, 1994·Baillière's Clinical Endocrinology and Metabolism·V A Randall
Dec 11, 1989·Biochimica Et Biophysica Acta·U Enderle-SchmittG Aumüller
Mar 28, 1996·International Journal of Cancer. Journal International Du Cancer·J P EloR Vihko
Dec 26, 1991·Annals of the New York Academy of Sciences·S ItamiS Takayasu

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