PMID: 9434131Jan 20, 1998Paper

Functional characterization and mRNA expression of pituitary adenylate cyclase activating polypeptide (PACAP) type I receptors in rat peritoneal macrophages

Biochimica Et Biophysica Acta
D PozoJ R Calvo

Abstract

The present work characterizes the mRNA expression of PACAP type I receptors in rat peritoneal macrophages but not in peritoneal lymphocytes by both retrotranscriptase and polymerase chain reaction (RT-PCR) and homologous Southern hybridization and the stimulation by PACAP27, PACAP38 and vasoactive intestinal peptide (VIP) of sn-1,2-diacylglycerol production in rat peritoneal macrophage membranes. The binding of [125I]PACAP27 was time and cell concentration dependent. Scatchard analysis of displacement of the bound tracer by unlabeled PACAP27 indicates the existence of two classes of binding sites. The dissociation constant (Kd) was 0.64 +/- 0.08 nM and the maximal binding capacity (Bmax) was 8.85 +/- 1.45 fmol/10(6) cells for the high affinity binding site. The low affinity binding site had a Kd of 0.10 +/- 0.06 microM with a Bmax of 300 +/- 21.9 fmol/10(6) cells. Scatchard analysis of VIP displacement data indicated the presence of two classes of binding sites with a Kd and Bmax different to those of PACAP27. These results suggest that PACAP binds to two binding sites, PACAP type I receptors and PACAP type II receptors. The PACAP27-stimulated diacylglycerol production was not affected by treatment with pertussis toxin. Howeve...Continue Reading

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Citations

Jun 23, 1999·Journal of Neuroimmunology·C MartinezJ Leceta
Jan 19, 2000·Immunology Today·D PozoJ R Calvo
Oct 31, 1998·Brain Research. Molecular Brain Research·F GillardonK A Hossmann
May 24, 2003·Trends in Molecular Medicine·David Pozo
Jan 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Carmen MartinezRosa P Gomariz
Jan 12, 2013·Current Protein & Peptide Science·Daria AugustyniakFionnuala T Lundy
Aug 31, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·David Pozo, Mario Delgado
Jan 22, 2008·Journal of Cellular Physiology·Yasu-Taka AzumaTadayoshi Takeuchi
Jun 1, 2004·Pharmacological Reviews·Mario DelgadoDoina Ganea
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Jun 14, 2019·Frontiers in Cellular and Infection Microbiology·Caio Andreeta FigueiredoIldiko Rita Dunay
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Feb 24, 2001·Annals of the New York Academy of Sciences·R GomarizM Delgado
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Sep 10, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Issam HarfiEric Sariban
Dec 27, 2008·Pharmacology & Therapeutics·Louise Dickson, Keith Finlayson

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