Functional characterization and transcriptional analysis of icd2 gene encoding an isocitrate dehydrogenase of Xanthomonas campestris pv. campestris

Archives of Microbiology
Ying-Chuan ChiangYi-Min Hsiao

Abstract

Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate. In the genome of Xanthomonas campestris pv. campestris, the phytopathogen that causes black rot in cruciferous plants, two putative IDH genes, icd1 and icd2, have been annotated. Their physiological roles in X. campestris pv. campestris are unclear. In this study, the icd2 gene from X. campestris pv. campestris was characterized in detail. We demonstrated genetically that icd2 gene encodes a functional IDH, and is involved in virulence as well as bacterial attachment. Furthermore, the icd2 transcription initiation site was mapped at nucleotide G, 127 nucleotide upstream of the icd2 translation start codon. In addition, promoter analysis revealed that icd2 expression exhibits a distinct expression profile under different culture conditions, is subjected to catabolite repression, and is affected by acetate. This is the first time that the function and transcription of icd2 have been characterized in the crucifer pathogen X. campestris pv. campestris.

References

Mar 16, 1979·Science·M Garnak, H C Reeves
Feb 1, 1992·Letters in Applied Microbiology·T W Wang, Y H Tseng
Apr 1, 1990·Applied and Environmental Microbiology·J F Fu, Y H Tseng
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J H HurleyR M Stroud
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Jan 1, 1993·Journal of Cellular Biochemistry·D C LaPorte
Sep 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·J Maxwell DowJi-Liang Tang
Oct 11, 2003·Biotechnology Advances·J W Chan, P H Goodwin
Oct 22, 2003·FEMS Microbiology Letters·Zheng-Xiang WangAlexander Steinbüchel
Jan 19, 2006·Extremophiles : Life Under Extreme Conditions·Shinsuke MakiYasuhiro Takada
Nov 21, 2009·FEMS Microbiology Reviews·Daniela Büttner, Ulla Bonas
Jun 16, 2010·Archives of Microbiology·Shougo MatsuoYasuhiro Takada
Apr 12, 2011·Nature Reviews. Microbiology·Robert P RyanJ Maxwell Dow
Feb 14, 2013·Genome Announcements·Stéphanie BolotLaurent D Noël
Dec 19, 2015·Genome Announcements·Yi-Chung LiuShan-Ho Chou

Datasets Mentioned

BETA
AE017340
CP007444
ALE70289.1

Methods Mentioned

BETA
PCR
Assay

Related Concepts

alpha-ketoglutaric acid, sodium salt
Bacterial Adhesion
Bacterial Proteins
Cauliflower (Dietary)
Isocitrate Dehydrogenase-I
Ketoglutaric Acids
Plant Diseases
Early Promoters, Genetic
Pathogenicity
Gene Expression Regulation, Bacterial

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.