Functional characterization of a basic D49 phospholipase A2 (LmTX-I) from the venom of the snake Lachesis muta muta (bushmaster)

Toxicon : Official Journal of the International Society on Toxinology
Daniela C S DamicoJosé Camillo Novello

Abstract

The whole venom of Lachesis muta muta is preponderantly neurotoxic but moderately myotoxic on the chick biventer cervicis preparation (BCp). We have now examined these toxic activities of a basic phospholipase A(2), LmTX-I, isolated from the whole venom. LmTX-I caused a significant concentration-dependent neuromuscular blockade in the BCp. The time to produce 50% neuromuscular blockade was 14.7+/-0.75 min (30 microg/ml), 23.6+/-0.9 min (10 microg/ml), 34+/-1.7 min (2.5 microg/ml) and 39.2+/-3.6 min (1 microg/ml), (n=5/concentration; p<0.05). Complete blockade with all tested concentrations was not accompanied by inhibition of the response to ACh. At the highest concentration, LmTX-I (30 microg/ml) significantly reduced contractures elicited by exogenous KCl (20mM), increased the release of creatine kinase (1542.5+/-183.9 IU/L vs 442.7+/-39.8 IU/L for controls after 120 min, p<0.05), and induced the appearance of degenerating muscle fibers ( approximately 15%). Quantification of myonecrosis indicated 14.8+/-0.8 and 2.0+/-0.4%, with 30 and 10 microg/mlvenom concentration, respectively, against 1.07+/-0.4% for control preparations. The findings indicate that the basic PLA(2) present on venom from L. m. muta (LmTX-I) possesses a do...Continue Reading

References

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Jun 23, 2005·Toxicon : Official Journal of the International Society on Toxinology·Daniela C S DamicoJosé Camillo Novello

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Citations

Mar 20, 2013·BioMed Research International·Victor Corasolla CarregariSergio Marangoni
Nov 11, 2008·Toxicon : Official Journal of the International Society on Toxinology·Tatiane FerreiraElen C T Landucci
May 27, 2008·Toxicon : Official Journal of the International Society on Toxinology·Andrana K CalgarottoSergio Marangoni
Jan 9, 2007·Toxicon : Official Journal of the International Society on Toxinology·Daniela C S DamicoCarla B Collares-Buzato
Jun 26, 2012·Journal of Biochemical and Molecular Toxicology·Lucas Benício CamposJosé Elpidio Barbosa
Nov 5, 2016·Toxicon : Official Journal of the International Society on Toxinology·Lourdes DiasStephen Hyslop
Nov 5, 2016·Toxicon : Official Journal of the International Society on Toxinology·Lourdes DiasStephen Hyslop
Apr 30, 2021·Toxicon : Official Journal of the International Society on Toxinology·Aline G Leão-TorresRafael S Floriano

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