PMID: 11902124Mar 21, 2002Paper

Functional characterization of a novel serotonin receptor (5-HTap2) expressed in the CNS of Aplysia californica

Journal of Neurochemistry
Demian BarbasLuc DesGroseillers

Abstract

Serotonin has been shown to be a neuromodulator in the Aplysia californica CNS. The diversity of serotonin actions is due to the existence of several different receptor subtypes. In this study we report the cloning of a full-length cDNA, coding for a novel serotonin receptor (5-HTap2). The receptor protein bears the characteristics of G protein-coupled receptors. It shares 68% and 34% of its amino acid sequence identity with the 5-HTlym receptor from Lymnaea stagnalis and the mammalian 5-HT1A receptor, respectively. When transfected in HEK 293 cells, 5-HTap2 was negatively coupled to adenylate cyclase. Ligand binding analysis indicated that the order of potencies of various drugs for the inhibition of [3H]LSD binding was: methiothepin > metergoline > 5-CT > PAPP > 5-HT > ketanserin > NAN-190 > 8-OH-DPAT > clozapine. RT-PCR amplification of RNA isolated from different tissues indicated that this receptor is expressed in the CNS and in bag cells. The expression of 5-HTap2 restricted to the CNS suggests an important role for this receptor in the modulation of neuronal functions in Aplysia. Moreover, the high expression of 5-HTap2 in the bag cells, associated with its pharmacological profile, suggests that this receptor may be impl...Continue Reading

Citations

Nov 1, 2007·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Stefan ClemensPaul S Katz
Nov 12, 2010·Amino Acids·Yanwei SunGert Lubec
Dec 20, 2003·Behavioural Brain Research·L Manuel-Apolinar, A Meneses
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yong-Seok LeeBong-Kiun Kaang
May 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·Carolyn M Sherff, Thomas J Carew
Mar 30, 2010·Cellular and Molecular Life Sciences : CMLS·Markus ThammWolfgang Blenau
Dec 13, 2005·Journal of Neurochemistry·Demian BarbasLuc DesGroseillers
Jun 4, 2008·Journal of Neuroscience Research·Ikue NagakuraWayne S Sossin
May 29, 2004·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Tobias Lehr, Rudolf Schipp
Sep 5, 2006·Biochimica Et Biophysica Acta·Chalermporn OngvarrasoponeSakol Panyim
Apr 16, 2002·Molecular Microbiology·Yuhong Wei, E B Newman
Mar 18, 2010·British Journal of Pharmacology·B TroppmannW Blenau
Jun 30, 2005·Archives of Insect Biochemistry and Physiology·Goudarz MolaeiAngela B Lange
Oct 23, 2010·Journal of Neurochemistry·Ikue NagakuraWayne S Sossin
Aug 6, 2005·The Journal of Comparative Neurology·Demian BarbasLuc DesGroseillers
May 14, 2020·Journal of Neurophysiology·Tyler R SizemoreAndrew M Dacks
Oct 6, 2018·The Journal of Experimental Biology·Ann Jane Tierney

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