Functional characterization of a UDP-glucose:flavonoid 3-O-glucosyltransferase from the seed coat of black soybean (Glycine max (L.) Merr.)

Phytochemistry
Nik KovinichBrian Miki

Abstract

The seed coats of black soybean (Glycine max (L.) Merr.) accumulate red (cyanidin-), blue (delphinidin-), purple (petunidin-), and orange (pelargonidin-based) anthocyanins almost exclusively as 3-O-glucosides; however, the responsible enzyme has not been identified. In this study, the full-length cDNA which encodes the enzyme that catalyzes the final step in anthocyanin biosynthesis, namely UDP-glucose:flavonoid 3-O-glucosyltransferase (UGT78K1), was isolated from the seed coat tissue of black soybean using rapid amplification of cDNA ends (RACE). Of the 28 flavonoid substrates tested, the purified recombinant protein glucosylated only anthocyanidins and flavonols, and demonstrated strict 3-OH regiospecificity. Galactose could also be transferred with relatively low activity to the 3-position of cyanidin or delphinidin in vitro. These findings are consistent with previous reports of mainly 3-O-glucosylated and minor amounts of 3-O-galactosylated anthocyanins in the seed coat of black soybean. The recombinant enzyme exhibited pronounced substrate inhibition by cyanidin at 100 microM acceptor concentration. Transfer of UGT78K1 into the Arabidopsis T-DNA mutant (ugt78d2) deficient in anthocyanidin and flavonol 3-O-glucosyltransfer...Continue Reading

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Citations

Apr 13, 2011·Journal of Agricultural and Food Chemistry·Takeshi SaitoMari Maeda-Yamamoto
Jul 21, 2011·Journal of Nucleic Acids·Lilian Cristina Baldon Aizza, Marcelo Carnier Dornelas
Jun 4, 2014·Carbohydrate Research·Ramesh Prasad PandeyJae Kyung Sohng
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Apr 10, 2013·Biotechnology and Bioengineering·Sailesh MallaJae Kyung Sohng
Apr 1, 2016·Applied Microbiology and Biotechnology·Bernd Hofer
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Nov 6, 2020·International Journal of Biological Macromolecules·Bing LiuRui Bao
Feb 13, 2021·International Journal of Molecular Sciences·Lingling XuanWei Zeng

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