Functional Characterization of a Hydroxyacid/Alcohol Hydroxycinnamoyl Transferase Produced by the Liverwort Marchantia emarginata

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Ping-Ping WangAi-Xia Cheng

Abstract

The aerial organs of most terrestrial plants are covered by a hydrophobic protective cuticle. The main constituent of the cuticle is the lipid polyester cutin, which is composed of aliphatic and aromatic domains. The aliphatic component is a polyester between fatty acid/alcohol and hydroxycinnamoyl acid. The BAHD/HxxxD family enzymes are central to the synthesis of these polyesters. The nature of this class of enzymes in bryophytes has not been explored to date. Here, a gene encoding a fatty ω-hydroxyacid/fatty alcohol hydroxycinnamoyl transferase (HFT) has been isolated from the liverwort Marchantia emarginata and has been functionally characterized. Experiments based on recombinant protein showed that the enzyme uses ω-hydroxy fatty acids or primary alcohols as its acyl acceptor and various hydroxycinnamoyl-CoAs-preferentially feruloyl-CoA and caffeoyl-CoA-as acyl donors at least in vitro. The transient expression of a MeHFT-GFP fusion transgene in the Nicotiana benthamiana leaf demonstrated that MeHFT is directed to the cytoplasm, suggesting that the feruloylation of cutin monomers takes place there.

References

Mar 11, 2000·Plant Physiology·N Dudareva, E Pichersky
Mar 7, 2002·Analytical Biochemistry·Till Beuerle, Eran Pichersky
Jun 18, 2002·Journal of Asian Natural Products Research·Hong-Xiang LouFeng-Qiang Wang
Jan 25, 2005·The Journal of Biological Chemistry·Xueyan MaJoachim Stöckigt
Apr 18, 2006·Current Opinion in Plant Biology·John C D'Auria
Dec 14, 2006·The Plant Journal : for Cell and Molecular Biology·John C D'AuriaJonathan Gershenzon
Feb 14, 2007·Macromolecular Bioscience·José Graça, Sara Santos
Apr 17, 2007·Current Opinion in Plant Biology·Rochus Franke, Lukas Schreiber
May 23, 2007·Nucleic Acids Research·Paul HortonKenta Nakai
Apr 29, 2008·Trends in Plant Science·Mike PollardJohn B Ohlrogge
Jul 12, 2008·Phytochemical Analysis : PCA·Giorgio GambinoIvana Gribaudo
Oct 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jin-Ying GouChang-Jun Liu
Jul 16, 2010·The Plant Journal : for Cell and Molecular Biology·Goro TaguchiNobuaki Hayashida
Jul 27, 2010·Trends in Plant Science·Lukas Schreiber
Apr 3, 2012·Current Opinion in Plant Biology·Fred BeissonMike Pollard

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Methods Mentioned

BETA
Assay
confocal microscopy

Software Mentioned

ZEN
Graphpad Prism
MEGA
WoLF PSORT

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