Functional Characterization of ABCC Proteins from Trypanosoma cruzi and Their Involvement with Thiol Transport

Frontiers in Microbiology
Kelli Monteiro da CostaJosé O Previato

Abstract

Chagas disease is a neglected disease caused by the protozoan Trypanosoma cruzi and affects 8 million people worldwide. The main chemotherapy is based on benznidazole. The efficacy in the treatment depends on factors such as the parasite strain, which may present different sensitivity to treatment. In this context, the expression of ABC transporters has been related to chemotherapy failure. ABC transporters share a well-conserved ABC domain, responsible for ATP binding and hydrolysis, whose the energy released is coupled to transport of molecules through membranes. The most known ABC transporters are ABCB1 and ABCC1, involved in the multidrug resistance phenotype in cancer, given their participation in cellular detoxification. In T. cruzi, 27 ABC genes were identified in the genome. Nonetheless, only four ABC genes were characterized: ABCA3, involved in vesicular trafficking; ABCG1, overexpressed in strains naturally resistant to benznidazole, and P-glycoprotein 1 and 2, whose participation in drug resistance is controversial. Considering P-glycoprotein genes are related to ABCC subfamily in T. cruzi according to the demonstration using BLASTP alignment, we evaluated both ABCB1-like and ABCC-like activities in epimastigote and ...Continue Reading

References

Nov 11, 1976·Biochimica Et Biophysica Acta·R L Juliano, V Ling
Jan 1, 1987·Transactions of the Royal Society of Tropical Medicine and Hygiene·L S Filardi, Z Brener
Apr 1, 1986·The Journal of Clinical Investigation·L M SlaterS Gupta
Jan 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·B Rotman, B W Papermaster
Aug 1, 1994·Experimental Parasitology·B DallagiovannaF Gamarro
Jan 1, 1996·Molecular and Biochemical Parasitology·B DallagiovannaS Castanys
Mar 14, 2001·Anais Da Academia Brasileira De Ciências·V M RumjanekM A Capella
Apr 18, 2001·The Journal of Biological Chemistry·D LégaréM Ouellette
Jan 31, 2002·Annual Review of Medicine·Michael M Gottesman
Mar 23, 2005·Biochemical and Biophysical Research Communications·José María Araújo-SantosFrancisco Gamarro
Apr 5, 2005·Immunology·Juliana Echevarria-LimaVivian M Rumjanek
Apr 23, 2005·Toxicology and Applied Pharmacology·Elaine M LeslieSusan P C Cole
May 27, 2005·Toxicology Letters·Sanjay Kumar, Uday Bandyopadhyay
Dec 13, 2005·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Susan PrattAnne H Dantzig
Jan 3, 2006·Trends in Cardiovascular Medicine·Thomas F SolbachOliver Zolk
Mar 23, 2006·Insect Biochemistry and Molecular Biology·Aurélio V Graça-SouzaPedro L Oliveira
May 10, 2006·Molecular and Biochemical Parasitology·Frédéric BringaudVirginie Coustou
Dec 6, 2007·Memórias do Instituto Oswaldo Cruz·José Rodrigues Coura
Apr 9, 2008·Biochimica Et Biophysica Acta·R Luise Krauth-Siegel, Marcelo A Comini
Oct 1, 2008·Experimental Parasitology·Fabiane Matos Dos SantosMaria Terezinha Bahia
May 12, 2009·Chemico-biological Interactions·Irving L M H AyeJeffrey A Keelan
May 26, 2009·Molecular and Biochemical Parasitology·Virginie SauvageIsabelle Villena
Jul 31, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Caryn Bern, Susan P Montgomery
Sep 24, 2009·Memórias do Instituto Oswaldo Cruz·Anis RassiJosé Antonio Marin-Neto
Aug 26, 2010·Current Drug Targets·Viridiana Olin-SandovalEmma Saavedra
Oct 5, 2011·Essays in Biochemistry·Elie Dassa
Oct 5, 2011·Essays in Biochemistry·Andrew J SlotSusan P C Cole
Oct 19, 2011·Journal of Parasitology Research·Marcia Cristina PaesJosé Roberto Meyer-Fernandes
Oct 26, 2011·Essays in Biochemistry·Dante A MaugeriJuan-José Cazzulo
May 29, 2012·Infectious Disease Clinics of North America·Anis RassiJoffre Marcondes de Rezende

❮ Previous
Next ❯

Citations

Jul 19, 2018·Pharmaceutics·Rosa Helena Bustos-CruzFernando Suárez
Oct 12, 2018·Anais Da Academia Brasileira De Ciências·Kelli M DA CostaVivian M Rumjanek
Nov 17, 2019·International Journal of Molecular Sciences·Rita CapelaFrancisca Lopes
May 31, 2018·International Journal of Oncology·Wei-Wei TongYong Liu
Nov 26, 2020·Parasitology Research·Fernanda Karoline Vieira da Silva TorchelsenMarta de Lana
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kelli Monteiro da CostaJosé Osvaldo Previato

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
flow cytometry

Software Mentioned

BLASTP
GraphPad Prism
Summit
HUGO
GraphPad

Related Concepts

© 2022 Meta ULC. All rights reserved