Functional characterization of Actinobacillus pleuropneumoniae urea transport protein, ApUT.

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Geeta GodaraJohn Mathai

Abstract

Urea transporters (UTs) effect rapid flux of urea across biological membranes. In the mammalian kidney, UT activity is essential for effective urine concentration. In bacteria, UT-mediated urea uptake permits intracellular urease to degrade urea to ammonia and CO(2), a process that either buffers acid loads or provides nutrient nitrogen. We have characterized the urea transport channel protein ApUT from Actinobacillus pleuropneumoniae. Kinetic analysis of bacterial inside-out membranes enriched in ApUT showed approximately 28-fold increase in urea permeability (3.3 +/- 0.4 x 10(-4) cm/s) compared with control vesicles (0.11 +/- 0.02 x 10(-4) cm/s). In addition to urea, ApUT also conducts water. Urea and water transport across the channel was phloretin and mercury inhibitable, and the site of inhibition may be located on the cytoplasmic side of the protein. Glycerol and urea analogs, such as methylamine, dimethylurea, formamide, acetamide, methylurea, propanamide, and ethylamine did not permeate across ApUT.

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Citations

Dec 7, 2013·Nature Communications·Reginald McNultyMartin B Ulmschneider
Mar 18, 2016·Journal of Phycology·Ramon TerradoConnie Lovejoy
May 5, 2012·The Biochemical Journal·Gerard H M HuysmansStephen A Baldwin
Feb 19, 2020·Biophysics Reviews·Shampa RaghunathanU Deva Priyakumar
May 17, 2017·Journal of Molecular Graphics & Modelling·Hai-Tian ZhangXiaoqin Zou

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