Functional Characterization of Cucurbitadienol Synthase and Triterpene Glycosyltransferase Involved in Biosynthesis of Mogrosides from Siraitia grosvenorii

Plant & Cell Physiology
Longhai DaiYuanxia Sun

Abstract

Mogrosides, the major bioactive components isolated from the fruits of Siraitia grosvenorii, are a family of cucurbitane-type tetracyclic triterpenoid saponins that are used worldwide as high-potency sweeteners and possess a variety of notable pharmacological activities. Mogrosides are synthesized from 2,3-oxidosqualene via a series of reactions catalyzed by cucurbitadienol synthase (CbQ), Cyt P450s (P450s) and UDP glycosyltransferases (UGTs) in vivo. However, the relevant genes have not been characterized to date. In this study, we report successful identification of SgCbQ and UGT74AC1, which were previously predicted via RNA-sequencing (RNA-seq) and digital gene expression (DGE) profile analysis of the fruits of S. grosvenorii. SgCbQ was functionally characterized by expression in the lanosterol synthase-deficient yeast strain GIL77 and was found to accumulate cucurbitadienol as the sole product. UGT74AC1 was heterologously expressed in Escherichia coli as a His-tag protein and it showed specificity for mogrol by transfer of a glucose moiety to the C-3 hydroxyl to form mogroside IE by in vitro enzymatic activity assays. This study reports the identification of CbQ and glycosyltransferase from S. grosvenorii for the first time...Continue Reading

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Citations

Sep 4, 2015·Chemical Society Reviews·Dong-Mei LiangJian-Jun Qiao
Nov 9, 2016·Proceedings of the National Academy of Sciences of the United States of America·Maxim ItkinArthur Schaffer
May 10, 2019·Critical Reviews in Biotechnology·Wentao SunChun Li
Feb 15, 2020·Natural Product Reports·Robert A Hill, Joseph D Connolly
Feb 14, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jing QiaoXiaojun Ma
Dec 12, 2018·Journal of Industrial Microbiology & Biotechnology·Jing QiaoZimian Ding
Jun 19, 2019·International Journal of Genomics·Jieying QianYaosheng Wu
Nov 6, 2018·Journal of Agricultural and Food Chemistry·Yu-Jia Zhao, Chun Li

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