Functional characterization of DEAD-box RNA helicases in Arabidopsis thaliana under abiotic stress conditions

Plant & Cell Physiology
Jin Sun KimHunseung Kang

Abstract

DEAD-box RNA helicases have been implicated to have a function during stress adaptation processes, but their functional roles in plant stress responses remain to be clearly elucidated. Here, we assessed the expression patterns and functional roles of two RNA helicases, AtRH9 and AtRH25, in Arabidopsis thaliana under abiotic stress conditions. The transcript levels of AtRH9 and AtRH25 were up-regulated markedly in response to cold stress, whereas their transcript levels were down-regulated by salt or drought stress. Phenotypic analysis of the transgenic plants and T-DNA-tagged mutants showed that the constitutive overexpression of AtRH9 or AtRH25 resulted in the retarded seed germination of Arabidopsis plants under salt stress conditions. AtRH25, but not AtRH9, enhanced freezing tolerance in Arabidopsis plants. Both AtRH9 and AtRH25 complemented the cold-sensitive phenotype of BX04 Escherichia coli mutant cells, but AtRH25 had much more prominent complementation ability than AtRH9. An in vitro nucleic acid binding assay showed that AtRH9 binds equally to all homoribopolymers, whereas AtRH25 binds preferentially to poly(G). Taken together, these results demonstrate that AtRH9 and AtRH25 impact on the seed germination of Arabidops...Continue Reading

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Citations

Nov 1, 2011·Plant Molecular Biology·Narendra TutejaRenu Tuteja
Feb 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Christopher E EllisonJohn W Taylor
Sep 6, 2012·Trends in Plant Science·Hunseung KangKyung Jin Kwak
Dec 18, 2014·Plant Signaling & Behavior·Simon BarakAsif Khan
Jul 16, 2015·Extremophiles : Life Under Extreme Conditions·Chenlin Liu, Xiaohang Huang
Nov 26, 2013·Plant Science : an International Journal of Experimental Plant Biology·Deok Hee YangHunseung Kang
Jun 20, 2018·Plant, Cell & Environment·Thomas FriedrichDaniel Schubert
Feb 5, 2019·Critical Reviews in Biotechnology·Sridevi NidumukkalaVenkateswara Rao Khareedu
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May 1, 2021·Viruses·Rosalba Rodriguez-PeñaHernan Garcia-Ruiz
Jul 3, 2021·International Journal of Molecular Sciences·Muthusamy MuthusamySoo-In Lee

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