PMID: 22362218Mar 1, 2012Paper

Functional characterization of double-knockout mouse sperm lacking SPAM1 and ACR or SPAM1 and PRSS21 in fertilization

The Journal of Reproduction and Development
Chong ZhouTadashi Baba

Abstract

Mammalian fertilization requires sperm to penetrate the cumulus to reach the oocyte. Although sperm hyaluronidase has long been believed to participate in the penetration process, our previous works revealed that neither of two sperm hyaluronidases, SPAM1 and HYAL5, are essential for fertilization. In this study, we have produced double-knockout mice lacking SPAM1 and either one of two sperm serine proteases, ACR and PRSS21, and characterized the mutant sperm. The SPAM1/ACR- and SPAM1/PRSS21-deficient males were fertile, whereas epididymal sperm of the mutant mice exhibited a reduced capacity to fertilize the oocytes in vitro. Despite normal motility, the ability of sperm to traverse the cumulus matrix was more severely impaired by the loss of SPAM1 and ACR or SPAM1 and PRSS21 than by the loss of only SPAM1. Moreover, SPAM1/ACR- and SPAM1/PRSS21-deficient sperm accumulated on the surface (outer edge) of the cumulus more abundantly than SPAM1-deficient sperm. These results suggest that ACR or PRSS21 or both may function cooperatively with SPAM1 in sperm/cumulus penetration.

References

Nov 9, 2001·Cell Structure and Function·L Zhuo, K Kimata
Jun 22, 2002·Science·Paul Primakoff, Diana G Myles
Oct 30, 2004·The Journal of Reproduction and Development·Ekyune KimTadashi Baba
Apr 20, 2005·Molecular and Cellular Endocrinology·Joanne S Richards
Nov 18, 2005·Analytical Biochemistry·Hans-Georg WisniewskiRobert Stern
Dec 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ekyune KimTadashi Baba
Jan 13, 2006·The Journal of Biological Chemistry·Ekyune KimTadashi Baba
Jul 24, 2008·The International Journal of Developmental Biology·Ekyune KimTadashi Baba
Aug 30, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Misuzu YamashitaTadashi Baba
Jul 17, 2009·Biology of Reproduction·Masanori KimuraTadashi Baba
Nov 21, 2009·The Journal of Reproduction and Development·Woojin KangTadashi Baba
Apr 7, 2010·The Journal of Clinical Investigation·Masahito IkawaMasaru Okabe
Mar 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Mayuko JinNoritaka Hirohashi

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Citations

Jan 11, 2016·Anatomical Science International·Chizuru Ito, Kiyotaka Toshimori
Oct 21, 2015·Systems Biology in Reproductive Medicine·Lakshminarayana SomashekarJanivara Parameswaraiah Ravindra
Jun 10, 2016·Human Reproduction Update·Elisabetta Tosti, Yves Ménézo
Sep 17, 2016·Journal of Proteome Research·Taylor PiniSimon P de Graaf
Nov 2, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Ali A Fouladi-NashtaGeraldine M Hartshorne
Aug 24, 2017·Molecular Reproduction and Development·Chong ZhouJian-Rong Jiang
Aug 8, 2020·Biology of Reproduction·Gagandeep Kaur Gahlay, Neha Rajput
Sep 2, 2017·Biology of Reproduction·Ayako IsotaniMasaru Okabe
Dec 19, 2012·Reproduction, Fertility, and Development·B M Gadella
Mar 10, 2017·Annals of Clinical and Translational Neurology·Mathieu MarellaPaula J Lapinskas
Sep 8, 2016·International Journal of Molecular Medicine·Katerina GeorgadakiVasilis Zoumpourlis

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