Functional characterization of Drosophila melanogaster peptide O-xylosyltransferase, the key enzyme for proteoglycan chain initiation and member of the core 2/I N-acetylglucosaminyltransferase family.

The Journal of Biological Chemistry
I B H Wilson

Abstract

Chondroitin and heparan sulfates are essential players in animal development and are synthesized by a series of glycosyltransferases, the first of which is UDP-alpha-D-xylose:proteoglycan core protein beta-D-xylosyltransferase (EC ). In the present study, a Drosophila melanogaster gene (CG17771), previously designated as a homologue of core 2 and I beta1,6-N-acetylglucosaminyltransferases, was shown to encode an active peptide O-xylosyltransferase. A novel coupled assay using matrix-assisted laser desorption ionization time-of-flight mass spectrometry demonstrated transfer of xylose to the peptide DDDSIEGSGGR. Analysis of sequences of various peptide O-xylosyltransferase and beta1,6-N-acetylglucosaminyltransferase sequences indicates that they are members of a large multifunctional protein family with a range of roles in beta-glycosylation of either peptide or glycan substrates. Because in contrast to mammals, there is only one fly peptide O-xylosyltransferase gene, it is anticipated that, given the key roles of proteoglycans, the hereby designated oxt gene is essential for viability.

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Citations

May 29, 2003·Biochimie·Monika BencúrováIain B H Wilson
Dec 5, 2002·Current Opinion in Structural Biology·Iain B H Wilson
Dec 30, 2006·The Journal of Biological Chemistry·Josef VoglmeirIain B H Wilson
Oct 1, 2008·Glycobiology·Kelly G ten HagenYing Zhang
Sep 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Rhoel R DinglasanMarcelo Jacobs-Lorena
Nov 23, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Bernard Moussian
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Apr 19, 2012·Natural Product Reports·Pierre Lafite, Richard Daniellou
Apr 9, 2019·Open Biology·Feifei ZhuKeping Chen
Apr 27, 2021·Organic & Biomolecular Chemistry·Jia GaoXuefei Huang

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