Functional characterization of Escherichia coli GlpG and additional rhomboid proteins using an aarA mutant of Providencia stuartii.

Journal of Bacteriology
Katy M ClemmerPhilip Rather

Abstract

The Providencia stuartii AarA protein is a member of the rhomboid family of intramembrane serine proteases and required for the production of an extracellular signaling molecule that regulates cellular functions including peptidoglycan acetylation, methionine transport, and cysteine biosynthesis. Additional aarA-dependent phenotypes include (i) loss of an extracellular yellow pigment, (ii) inability to grow on MacConkey agar, and (iii) abnormal cell division. Since these phenotypes are easily assayed, the P. stuartii aarA mutant serves as a useful host system to investigate rhomboid function. The Escherichia coli GlpG protein was shown to be functionally similar to AarA and rescued the above aarA-dependent phenotypes in P. stuartii. GlpG proteins containing single alanine substitutions at the highly conserved catalytic triad of asparagine (N154A), serine (S201A), or histidine (H254A) residues were nonfunctional. The P. stuartii aarA mutant was also used as a biosensor to demonstrate that proteins from a variety of diverse sources exhibited rhomboid activity. In an effort to further investigate the role of a rhomboid protein in cell physiology, a glpG mutant of E. coli was constructed. In phenotype microarray experiments, the gl...Continue Reading

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Citations

Mar 19, 2011·Cell Death and Differentiation·D V JeyarajuL Pellegrini
Oct 30, 2010·BMC Microbiology·David P KateeteMoses L Joloba
Nov 1, 2011·Genome Biology·Sinisa Urban, Seth W Dickey
Dec 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Adam Ben-ShemEitan Bibi
Jul 26, 2014·Annual Review of Cell and Developmental Biology·Matthew Freeman
Jan 29, 2013·Journal of Molecular Biology·Christelle Lazareno-SaezM Joanne Lemieux
Oct 20, 2011·Physiologia Plantarum·Ronit Rimon Knopf, Zach Adam
Mar 23, 2013·Biochimica Et Biophysica Acta·Philip Rather
Oct 9, 2013·Biochimica Et Biophysica Acta·Cory L Brooks, M Joanne Lemieux
Aug 28, 2012·Biochimica Et Biophysica Acta·Padmapriya SampathkumarM Joanne Lemieux
Mar 13, 2009·Structure·Ana-Nicoleta BondarStephen H White
Jan 12, 2007·Proceedings of the National Academy of Sciences of the United States of America·Lindsay G StevensonPhilip N Rather
Apr 28, 2020·The EMBO Journal·Guangyu LiuChristoph M Tang
Nov 14, 2018·The Journal of General Physiology·Kristen A Gaffney, Heedeok Hong
May 4, 2017·Biochimica Et Biophysica Acta. Molecular Cell Research·Stefan DüsterhöftMatthew Freeman

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