Functional characterization of human CD34+ cells that express low or high levels of the membrane antigen CD111 (nectin 1)

Leukemia
Ghania BelaalouiChristian Chabannon

Abstract

Nectins are recently described adhesion molecules that are widely expressed on many tissues, including the hematopoietic tissue. Nectin 1 (CD111) is expressed on a higher proportion of mobilized peripheral blood (mPB) than cord blood (CB) CD34+ cells, and of CD34+/CD38+ cells when compared with CD34+/CD38- cells. We studied functional properties of human CB and mPB CD34+ cells that express low or high levels of CD111. CD34+/CD111(dim) cells contain a higher proportion of cells in G0/G1 phase than CD34+/CD111(bright) cells. CD34+/CD111(bright) cells contain more erythroid progenitors: CFU-E, than their counterparts, which on the opposite contain more HPP-CFC. Limiting dilution analyses demonstrate a higher frequency of immature progenitors: cobblestone-area colony-forming cells, CD34+/CD111(dim) than in CD34+/CD111(bright) cells. In vitro differentiation assays demonstrate a higher frequency of B-, T- and dendritic-cell precursors, but less NK-cell precursors in CD34+/CD111(dim) cells. Evaluation of engraftment in NOD-SCID mice shows that SCID repopulating cells are more frequent among mPB CD34+/CD111(dim) cells. Liquid culture of CD34+/CD111(dim) cells with erythropoietin shows that CD111 expression increases simultaneously wit...Continue Reading

References

Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C M BaumB Peault
Feb 1, 1990·The Journal of Experimental Medicine·K MiyakeP W Kincade
Oct 1, 1995·The Journal of Cell Biology·S ArmeanuG Klein
Sep 3, 1998·The Journal of Clinical Investigation·J MollP Herrlich
Feb 2, 2002·Experimental Hematology·Sigrid P M A OomenIvo P Touw
Feb 6, 2002·The Journal of Cell Biology·Akira MizoguchiYoshimi Takai

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