Functional characterization of human T cells immortalized by oncogene transfection.

Cytotechnology
S AlamS Shirahata

Abstract

We have succeeded in immortalizing human lymphocytes derived from the peripheral blood of a healthy donor and of an atopic patient, and from the lymph node of a cancer patient by oncogene transfection (Alam et al., 1996). All immortalized human lymphocytes were shown to be CD3+ and CD19-, indicating that these immortalized human lymphocytes were all T cells. We established 317, 154 and 692 individual immortalized human T cell lines derived from the healthy donor, the atopic patient and the cancer patient, respectively. The ratios of CD4+ and CD8+ subpopulations within the set containing immortalized T cells derived from the healthy donor were shown to be varied depending on the combinations of transfected oncogenes used. However, CD8+ cells were found to be the dominant subpopulation of immortalized T cells derived from the atopic patient and the cancer patient. These immortalized T cells showed different proliferative responses in the presence of exogenous human IL-2 depending on their origin, and was consistent with the surface expression of the IL-2 receptor. Furthermore, the cytokine secretion patterns of these immortalized T cells stimulated with mitogen were investigated. The results showed that the immortalized T cells f...Continue Reading

References

Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B BiesingerB Fleckenstein
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·P PisaR Kiessling
Jan 1, 1993·Cytotechnology·F OkanoH Murakami
Feb 1, 1994·International Journal of Cancer. Journal International Du Cancer·B TroidlE Gebhart

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