Functional characterization of MigA and WapR: putative rhamnosyltransferases involved in outer core oligosaccharide biosynthesis of Pseudomonas aeruginosa

Journal of Bacteriology
Karen K H PoonJoseph S Lam

Abstract

Pseudomonas aeruginosa lipopolysaccharide (LPS) contains two glycoforms of core oligosaccharide (OS); one form is capped with O antigen through an alpha-1,3-linked L-rhamnose (L-Rha), while the other is uncapped and contains an alpha-1,6-linked L-Rha. Two genes in strain PAO1, wapR (PA5000) and migA (PA0705), encode putative glycosyltransferases associated with core biosynthesis. We propose that WapR and MigA are the rhamnosyltransferases responsible for the two linkages of L-Rha to the core. Knockout mutants with mutations in both genes were generated. The wapR mutant produced LPS lacking O antigen, and addition of wapR in trans complemented this defect. The migA mutant produced LPS with a truncated outer core and showed no reactivity to outer core-specific monoclonal antibody (MAb) 5C101. Complementation of this mutant with migA restored reactivity of the LPS to MAb 5C101. Interestingly, LPS from the complemented migA strain was not reactive to MAb 18-19 (specific for the core-plus-one O repeat). This was due to overexpression of MigA in the complemented strain that caused an increase in the proportion of the uncapped core OS, thereby decreasing the amount of the core-plus-one O repeat, indicating that MigA has a regulatory r...Continue Reading

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Citations

Aug 4, 2010·Antimicrobial Agents and Chemotherapy·Carolina Alvarez-OrtegaJosé Luis Martínez
Oct 17, 2012·Antimicrobial Agents and Chemotherapy·Lucía FernándezRobert E W Hancock
Feb 2, 2010·Journal of Bacteriology·Thilo KöhlerChristian van Delden
Oct 18, 2011·Biochemistry. Biokhimii︠a︡·D Kocíncová, J S Lam
May 17, 2011·BMC Microbiology·Julia GarbeMax Schobert
Jul 21, 2009·Carbohydrate Research·Kerry Hosmer Caffall, Debra Mohnen
Aug 28, 2009·Innate Immunity·Jerry D KingJoseph S Lam
Nov 17, 2020·Frontiers in Bioengineering and Biotechnology·Andreas Wittgens, Frank Rosenau
Feb 19, 2021·The Biochemical Journal·Ben A WagstaffHelge C Dorfmueller
Mar 30, 2021·Frontiers in Bioengineering and Biotechnology·Mélanie GrosjeanPaulo Juarez

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