Abstract
Neurons are known to express a high-affinity Na+ -coupled dicarboxylate transporter(s) for uptake of tricarboxylic acid cycle intermediates, such as alpha-ketoglutarate and malate, which are precursors for neurotransmitters including glutamate and gamma-aminobutyric acid. There is, however, little information available on the molecular identity of the transporters responsible for this uptake process in neurons. In the present study, we investigated the characteristics of Na+ -dependent citrate transport in primary cultures of neurons from mouse cerebral cortex and established the molecular identity of this transport system as the Na+ -coupled citrate transporter (NaC2/NaCT). Reverse transcriptase (RT)-PCR and immunocytochemical analyses revealed that only NaC2/NaCT was expressed in mouse cerebrocortical neurons but not in astrocytes. Uptake of citrate in neurons was Na+ -dependent, Li+ -sensitive, and saturable with the Kt value of 12.3 microM. This Kt value was comparable with that in the case of Na+ -dependent succinate transport (Kt = 9.2 microM). Na+ -activation kinetics revealed that the Na+ -to-citrate stoichiometry was 3.4:1 and concentration of Na+ necessary for half-maximal activation (K0.5(Na)) was 45.7 mM. Na+ -depen...Continue Reading
References
Oct 1, 1992·Journal of Neuroscience Research·L HertzA Schousboe
Jan 1, 1991·Neurochemical Research·L A PengL Hertz
Jul 22, 1991·Neuroscience Letters·U SonnewaldA Schousboe
Mar 11, 1985·Brain Research·R P ShankG L Campbell
May 1, 1982·Neurochemical Research·R P Shank, G L Campbell
Apr 1, 1984·Journal of Neurochemistry·R P Shank, G L Campbell
Feb 23, 1981·Life Sciences·R P Shank, G L Campbell
Nov 1, 1983·Journal of Neurochemistry·A C YuA Schousboe
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·N WestergaardA Schousboe
Jan 1, 1993·Developmental Neuroscience·J C LehmannR P Shank
Jul 18, 1994·Neuroscience Letters·N WestergaardA Schousboe
Jan 1, 1995·Developmental Neuroscience·N WestergaardA Schousboe
Sep 23, 1997·Glia·A SchousboeU Sonnewald
Aug 8, 1998·The Journal of Biological Chemistry·X Z ChenM A Hediger
Jan 28, 1999·The Journal of Biological Chemistry·R KekudaV Ganapathy
Apr 1, 1999·Annual Review of Physiology·A M Pajor
May 17, 2000·The Journal of Membrane Biology·A M Pajor
Aug 15, 2002·The Journal of Biological Chemistry·Katsuhisa InoueVadivel Ganapathy
Nov 26, 2002·Biochemical and Biophysical Research Communications·Katsuhisa InoueVadivel Ganapathy
Jun 27, 2003·The Biochemical Journal·Katsuhisa InoueVadivel Ganapathy
Aug 14, 2003·Pflügers Archiv : European journal of physiology·Daniel Markovich, Heini Murer
Dec 6, 2003·The Biochemical Journal·Katsuhisa InoueVadivel Ganapathy
Mar 19, 2005·Neuroscience Letters·Kazunari TanakaTakuya Fujita
Apr 20, 2005·Journal of Neurochemistry·Takuya FujitaVadivel Ganapathy
May 3, 2005·Brain Research·Miyuki WadaTakuya Fujita
Citations
Mar 16, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Franziska StellmerChris Mühlhausen
Oct 12, 2013·Pflügers Archiv : European journal of physiology·Ana M Pajor
Apr 12, 2008·Journal of Inherited Metabolic Disease·C MühlhausenT Braulke
Jan 26, 2011·Journal of Inherited Metabolic Disease·Katja BrauburgerBirgitta C Burckhardt
Jul 3, 2009·Zhurnal evoliutsionnoĭ biokhimii i fiziologii·D A Aliverdieva, D V Mamaev
Jan 6, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Wing-Hang Tong, Tracey A Rouault
Mar 20, 2013·Molecular Aspects of Medicine·M J BergeronD Markovich
Sep 29, 2011·Micron : the International Research and Review Journal for Microscopy·Hanmei QiuPeng Liu
Sep 20, 2015·Brain : a Journal of Neurology·Katia HardiesUNKNOWN autosomal recessive working group of the EuroEPINOMICS RES Consortium
Jan 1, 2013·Computational and Structural Biotechnology Journal·Diana M Willmes, Andreas L Birkenfeld
Jan 15, 2008·Journal of Neuroscience Research·Tünde MolnárJulianna Kardos
Dec 4, 2013·The Journal of Biological Chemistry·Leopold AdlerYiannis Koutalos
Jan 27, 2015·The Journal of Pharmacology and Experimental Therapeutics·Elangovan GopalVadivel Ganapathy
Dec 15, 2007·Neurochemical Research·Alexander ZlotnikVivian I Teichberg
Sep 16, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Elangovan GopalVadivel Ganapathy
Dec 29, 2019·Pharmacological Reviews·Tina SchumannAndreas L Birkenfeld
Aug 18, 2018·Cancer Research·Maria E Mycielska, Edward K Geissler
Jul 20, 2020·Neurobiology of Disease·Christine HenkeWolfgang Löscher