PMID: 9171168May 1, 1997Paper

Functional characterization of NGF-secreting cell grafts to the acutely injured spinal cord

Cell Transplantation
M H TuszynskiI Miller

Abstract

Previously we reported that grafts of cells genetically modified to produce human nerve growth factor (hNGF) promoted specific and robust sprouting of spinal sensory, motor, and noradrenergic axons. In the present study we extend these investigations to assess NGF effects on corticospinal motor axons and on functional outcomes after spinal cord injury. Fibroblasts from adult rats were transduced to express human NGF; control cells were not genetically modified. Fibroblasts were then grafted to sites of midthoracic spinal cord dorsal hemisection lesions. Three months later, recipients of NGF-secreting grafts showed deficits on conditioned locomotion over a wire mesh that did not differ in extent from control-lesioned animals. On histological examination, NGF-secreting grafts elicited specific sprouting from spinal primary sensory afferent axons, local motor axons, and putative cerulospinal axons as previously reported, but no specific responses from corticospinal axons. Axons responding to NGF robustly penetrated the grafts but did not exit the grafts to extend to normal innervation territories distal to grafts. Grafted cells continued to express NGF protein through the experimental period of the study. These findings indicate t...Continue Reading

Citations

Jul 3, 1999·Journal of Neurotrauma·M H TuszynskiS Szollar
May 15, 2001·The Journal of Physiology·L L JonesM H Tuszynski
Apr 2, 2004·Journal of Neuropathology and Experimental Neurology·Yu-Shang LeeVernon W Lin
Nov 13, 2002·Journal of Neurotrauma·Yu-Shang LeeVernon W Lin
Oct 7, 2003·Annual Review of Biomedical Engineering·Christine E Schmidt, Jennie Baier Leach
Dec 22, 2010·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Martin M MortazaviNicholas Theodore
Jul 20, 2012·Continuum : Lifelong Learning in Neurology·Mark A Livecchi
Jun 9, 1998·Annals of the New York Academy of Sciences·M H Tuszynski
Feb 13, 2001·Experimental Neurology·D J LieblL F Parada
Jan 30, 2004·The Spine Journal : Official Journal of the North American Spine Society·Nicholas C Bambakidis, Robert H Miller
Nov 8, 2005·Progress in Neurobiology·Ronald DeumensElbert A J Joosten

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