Functional characterization of OsRacB GTPase--a potentially negative regulator of basal disease resistance in rice

Plant Physiology and Biochemistry : PPB
Young-Ho JungNam-Soo Jwa

Abstract

The rice genome contains at least seven expressed Rop small GTPase genes. Of these Rops, OsRac1 is the only characterized gene that has been implicated in disease resistance as a positive regulator. To our interest in finding a negative ROP regulator of disease resistance in rice, we applied a "phylogeny of function" approach to rice Rops, and identified OsRacB based on its close genetic orthologous relationship with the barley HvRacB gene, a known negative regulator of disease resistance. To determine the function of OsRacB, we isolated the OsRacB cDNA and conducted gene expression and transgenic studies. OsRacB, a single copy gene in the genome of rice, shared 98% identity with HvRacB at the amino acid level. Its mRNA was strongly expressed in leaf sheath (LS) and in panicles, but was very weakly expressed in young and mature leaves. The basal mRNA level of OsRacB in LS of two-week-old seedlings was strongly down-regulated upon wounding by cut and treatment with jasmonic acid. A dramatic down-regulation in the OsRacB transcripts was also found in plants inoculated with the blast pathogen, Magnaporthe grisea. Interestingly, transgenic rice plants over-expressing OsRacB showed increased symptom development in response to rice b...Continue Reading

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Citations

Sep 10, 2013·International Journal of Molecular Sciences·Birsen Cevher-Keskin
Jul 9, 2014·Annual Review of Phytopathology·Chris C N van Schie, Frank L W Takken
Jul 18, 2015·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Jing SuXiaoyuan Zhu
Oct 30, 2014·Frontiers in Plant Science·Yoji KawanoKo Shimamoto
Jan 8, 2020·International Journal of Molecular Sciences·Na-Hyun ShinSoo-Cheul Yoo
Oct 2, 2009·Plant Signaling & Behavior·Bayram Yuksel, Abdul R Memon
Sep 15, 2021·Development, Growth & Differentiation·Yangfan XuWanqi Liang

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