Functional characterization of PaLAX1, a putative auxin permease, in heterologous plant systems

Plant Physiology
Klára HoyerováEva Zazímalová

Abstract

We have isolated the cDNA of the gene PaLAX1 from a wild cherry tree (Prunus avium). The gene and its product are highly similar in sequences to both the cDNAs and the corresponding protein products of AUX/LAX-type genes, coding for putative auxin influx carriers. We have prepared and characterized transformed Nicotiana tabacum and Arabidopsis thaliana plants carrying the gene PaLAX1. We have proved that constitutive overexpression of PaLAX1 is accompanied by changes in the content and distribution of free indole-3-acetic acid, the major endogenous auxin. The increase in free indole-3-acetic acid content in transgenic plants resulted in various phenotype changes, typical for the auxin-overproducing plants. The uptake of synthetic auxin, 2,4-dichlorophenoxyacetic acid, was 3 times higher in transgenic lines compared to the wild-type lines and the treatment with the auxin uptake inhibitor 1-naphthoxyacetic acid reverted the changes caused by the expression of PaLAX1. Moreover, the agravitropic response could be restored by expression of PaLAX1 in the mutant aux1 plants, which are deficient in auxin influx carrier activity. Based on our data, we have concluded that the product of the gene PaLAX1 promotes the uptake of auxin into c...Continue Reading

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Citations

Mar 20, 2010·Cold Spring Harbor Perspectives in Biology·Eva ZazímalováPetr Hosek
Oct 15, 2014·Plant, Cell & Environment·Heming ZhaoJie Zhao
Jan 4, 2012·The Plant Journal : for Cell and Molecular Biology·Richard J Pattison, Carmen Catalá
Dec 17, 2009·The Plant Journal : for Cell and Molecular Biology·Adriana JelínkováJan Petrásek
May 7, 2014·The Plant Journal : for Cell and Molecular Biology·Yanxia XuDe An Jiang
May 27, 2016·The Plant Journal : for Cell and Molecular Biology·Justine Braguy, Matias D Zurbriggen
Jun 16, 2009·Phytochemistry·Andrzej Bajguz, Alicja Piotrowska
May 12, 2021·Physiologia Plantarum·Gabriella FrosiMauro Guida Santos
Jul 28, 2009·Development·Jan Petrásek, Jirí Friml

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