Functional Characterization of Phalaenopsis aphrodite Flowering Genes PaFT1 and PaFD

PloS One
Seonghoe JangElmon Schmelzer

Abstract

We show that the key flowering regulators encoded by Phalaenopsis aphrodite FLOWERING LOCUS T1 (PaFT1) and PaFD share high sequence homologies to these from long-day flowering Arabidopsis and short-day flowering rice. Interestingly, PaFT1 is specifically up-regulated during flowering inductive cooling treatment but is not subjected to control by photoperiod in P. aphrodite. Phloem or shoot apex-specific expression of PaFT1 restores the late flowering of Arabidopsis ft mutants. Moreover, PaFT1 can suppress the delayed flowering caused by SHORT VEGATATIVE PHASE (SVP) overexpression as well as an active FRIGIDA (FRI) allele, indicating the functional conservation of flowering regulatory circuit in different plant species. PaFT1 promoter:GUS in Arabidopsis showed similar staining pattern to that of Arabidopsis FT in the leaves and guard cells but different in the shoot apex. A genomic clone or heat shock-inducible expression of PaFT1 is sufficient to the partial complementation of the ft mutants. Remarkably, ectopic PaFT1 expression also triggers precocious heading in rice. To further demonstrate the functional conservation of the flowering regulators, we show that PaFD, a bZIP transcription factor involved in flowering promotion, ...Continue Reading

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Citations

Nov 20, 2018·Journal of Experimental Botany·Anna B DommesAnnette Becker
Nov 2, 2019·International Journal of Molecular Sciences·Yi-Jyun LinSwee-Suak Ko
Dec 23, 2017·Plant Cell Reports·Kah Wee KohMing-Tsair Chan
Dec 6, 2019·Frontiers in Plant Science·Zhi Wei Norman TeoLisha Shen
Apr 28, 2017·Plant Signaling & Behavior·Hsin-Mei WangSeonghoe Jang
Oct 28, 2019·Frontiers in Plant Science·Shan-Li WangFure-Chyi Chen
Dec 12, 2020·Frontiers in Plant Science·Kana KurokawaMasumi Yamagishi
Jul 29, 2021·Plant Science : an International Journal of Experimental Plant Biology·Fangfang CaiJiaqi Zhang

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Datasets Mentioned

BETA
BAA77838
BAA77840
AB027456
ADI58462
ACX85427
ABJ97441
ABW73562
BAK14369
AB161112
ACC59806

Methods Mentioned

BETA
transgenic
PCR
Two-Hybrid
cDNA
reverse transcription PCR
chemical sprays

Software Mentioned

MEGA
MEGA5
clustalW
PaACT

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