Functional characterization of receptors with affinity for PYY, NPY, [Leu31,Pro34]NPY and PP in a human colonic epithelial cell line

British Journal of Pharmacology
Helen M Cox, Iain R Tough

Abstract

1. Confluent epithelial layers of a human adenocarcinoma cell line called Colony-6 have been shown to respond to nanomolar concentrations of vasoactive intestinal polypeptide (VIP), peptide YY (PYY), neuropeptide Y (NPY) and somatostatin (Som). 2. The VIP-induced increase in basal short-circuit current (SCC) was attenuated by basolateral application of Som, PYY or NPY, and also by the Y1-receptor agonist [Leu31,Pro34]NPY, as well as pancreatic polypeptide (PP). High concentrations (0.1-3.0 microM) of NPY(2-36) were effective but the C-terminal fragment NPY(13-36) (0.1-1.0 microM) and desamidoNPY (0.6 microM) were not active. A rank order of agonist EC50 values was: PYY > NPY > [Leu31,Pro34]NPY > PP > NPY(2-36) > NPY (13-36). 3. Receptors for all these peptides were preferentially located within the basolateral domain. Apical addition of PP (1 microM) and Som (100 nM) had no effect upon basal SCC while apical VIP (10 nM) responses were 18%, and apical PYY (100 nM) were 27% the size of respective basolateral controls (100%). 4. Cross-desensitization was observed between [Leu31,Pro34]NPY (1 microM) and both PYY (100 nM) and PP (1 microM) and between PYY and NPY(2-36) (1 microM), but was not significant between PYY (100 nM) and PP ...Continue Reading

References

Jan 1, 1992·Journal of Cancer Research and Clinical Oncology·F van ValenH Jürgens
Oct 1, 1991·Trends in Pharmacological Sciences·M C Michel
Aug 10, 1990·European Journal of Pharmacology·J A FerrarH M Cox
Aug 1, 1991·Gastroenterology·U Holzer-PetscheG J Krejs
Nov 1, 1988·Pflügers Archiv : European journal of physiology·H M Cox, A W Cuthbert
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R GilbertR L Gingerich
May 1, 1994·Trends in Pharmacological Sciences·L Grundemar, R Håkanson
Dec 1, 1994·British Journal of Pharmacology·H M Cox, I R Tough

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Citations

Apr 1, 1997·Diseases of the Colon and Rectum·C D LiuD W McFadden
Feb 5, 2002·Peptides·Magdy El-SalhyAke Danielsson
Oct 18, 2002·Peptides·Laurent FerrierHervé M Blottiere
Aug 15, 1998·Molecular and Cellular Endocrinology·C Robin-JagerschmidtC Bénicourt
Jan 13, 2001·British Journal of Pharmacology·H M CoxN D Holliday
Mar 22, 2002·British Journal of Pharmacology·Helen M Cox, Iain R Tough
Oct 5, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Seema SaksenaPradeep K Dudeja
Jul 30, 2014·Angewandte Chemie·Veronika MädeAnnette G Beck-Sickinger
Feb 13, 2014·British Journal of Pharmacology·Stephen P H AlexanderUNKNOWN CGTP Collaborators
Dec 14, 2001·The Journal of Biological Chemistry·Hervé GicquiauxJean-Luc Galzi
Dec 1, 1996·Trends in Pharmacological Sciences·R J Playford, H M Cox

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