Functional characterization of rose phenylacetaldehyde reductase (PAR), an enzyme involved in the biosynthesis of the scent compound 2-phenylethanol

Journal of Plant Physiology
Xiao-Min ChenNaoharu Watanabe

Abstract

2-Phenylethanol (2PE) is a prominent scent compound released from flowers of Damask roses (Rosa×damascena) and some hybrid roses (Rosa 'Hoh-Jun' and Rosa 'Yves Piaget'). 2PE is biosynthesized from l-phenylalanine (l-Phe) via the intermediate phenylacetaldehyde (PAld) by two key enzymes, aromatic amino acid decarboxylase (AADC) and phenylacetaldehyde reductase (PAR). Here we describe substrate specificity and cofactor preference in addition to molecular characterization of rose-PAR and recombinant PAR from R.×damascena. The deduced amino acid sequence of the full-length cDNA encoded a protein exhibiting 77% and 75% identity with Solanum lycopersicum PAR1 and 2, respectively. The transcripts of PAR were higher in petals than calyxes and leaves and peaking at the unfurling stage 4. Recombinant PAR and rose-PAR catalyzed reduction of PAld to 2PE using NADPH as the preferred cofactor. Reductase activity of rose-PAR and recombinant PAR were higher for aromatic and aliphatic aldehydes than for keto-carbonyl groups. Both PARs showed that (S)-[4-(2)H] NADPH was preferentially used over the (R)-[4-(2)H] isomer to give [1-(2)H]-2PE from PAld, indicating that PAR can be classified as short-chain dehydrogenase reductase (SDR).

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Citations

Nov 3, 2009·Plant Molecular Biology·Moran FarhiAlexander Vainstein
Nov 21, 2012·BMC Genomics·Annick DuboisMohammed Bendahmane
Feb 3, 2016·Scientific Reports·Hiroshi HirataNaoharu Watanabe
Aug 14, 2012·Biosensors & Bioelectronics·I BazinC Gonzalez
Mar 24, 2015·Fungal Genetics and Biology : FG & B·Shunsuke MasuoNaoki Takaya
Feb 5, 2015·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Akbar KaramiHassan Salehi
Jun 15, 2016·Bioscience, Biotechnology, and Biochemistry·Hiroshi HirataNaoharu Watanabe
Aug 1, 2016·Trends in Plant Science·Pulu SunSylvie Baudino
Aug 27, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fang DongZiyin Yang
Mar 5, 2014·Plant, Cell & Environment·Joëlle K MuhlemannNatalia Dudareva
Oct 19, 2017·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Noriko NakamuraYoshikazu Tanaka
Mar 7, 2020·The FEBS Journal·Elesha R HoffarthKatherine S Ryan
May 10, 2019·Horticulture Research·Marinus J M SmuldersFabrice Foucher
Dec 2, 2020·International Journal of Molecular Sciences·Jesús Picazo-AragonésFrancisco Balao

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