Functional characterization of starvation-induced lysosomal activity in Saccharomyces cerevisiae

Applied Microbiology and Biotechnology
Jihee YoonJiho Min

Abstract

Starvation induces significant alterations in lysosomal enzymes, and reduced concentrations of glucose increases the activity of several lysosomal enzymes. Therefore, to evaluate the lysosomal antimicrobial activity under starvation conditions, we added 0, 5, 10, 20, or 40 g/l of glucose (0%, 0.5%, 1%, 2%, or 4% glucose) supplemented YP medium to cultured Saccharomyces cerevisiae, and lysosomal fractions were isolated from S. cerevisiae grown under the various culture conditions. The lysosomes isolated from each condition exhibited increased antimicrobial activity against Escherichia coli as determined by a decrease in glucose concentration. In addition, a starvation-dependent increase in lysosomal activity coincided with increased lysosome intensity at the cytosol and distinct protein expression from lysosomes in S. cerevisiae. It also was determined found that the lysosomes have antimicrobial activity against seven different microorganisms, including E. coli, and starvation-induced lysosomes showed enhanced antimicrobial activity compared to those from normal lysosomes. These results suggest the possibility that lysosomal alterations during starvation may induce conditions that activate lysosomes for future development of eff...Continue Reading

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Apr 17, 2009·The New England Journal of Medicine·Daniel J Klionsky
Dec 10, 2009·Journal of Microbiology and Biotechnology·Jihee YoonJiho Min

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Citations

Feb 20, 2016·Molecular and Cellular Biochemistry·Le Minh TranJiho Min
Nov 12, 2014·Environmental Health and Toxicology·Ngoc-Tu NguyenJiho Min
Oct 6, 2016·Enzyme and Microbial Technology·Le Minh TranJiho Min
Feb 12, 2020·Journal of Basic Microbiology·Dong J ParkJiho Min
May 26, 2016·Molecular and Cellular Biochemistry·Jihee YoonJiho Min
Jul 28, 2021·Enzyme and Microbial Technology·Dong-Jun ParkJiho Min

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