Functional characterization of the atopy-associated gene PHF11

The Journal of Allergy and Clinical Immunology
Emily ClarkeGraham J Jones

Abstract

Polymorphisms in the plant homeodomain finger protein 11 gene (PHF11) are associated with increased total serum IgE levels, asthma, and severe atopic dermatitis (AD) in children. Although PHF11 includes a plant homeodomain, a motif often found in transcriptional regulators, the function of PHF11 has not been investigated. We sought to test (1) whether PHF11 regulates the transcription of genes involved in allergic disorders and (2) whether polymorphisms in PHF11 predict changes in the expression or function of this gene. Microarray analysis was used to examine the expression of PHF11 in different immune cell subsets, and the function of PHF11 was tested by using small interfering RNA-induced knockdown or overexpression of PHF11 in primary CD4+ T cells or Jurkat T cells. Genotype-dependent effects on PHF11 expression were tested by using an allele-specific gene expression, and the transcriptional activity of PHF11 was determined by using luciferase hybrid gene reporter assays and in vitro DNA-binding electromobility shift assays. PHF11 expression was higher in T(H)1 cells relative to that in T(H)2 cells, and knockdown of PHF11 expression reduced expression of the T(H)1-type cytokines IFN-gamma and IL-2. The G-allele of a 3' untr...Continue Reading

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Citations

Apr 28, 2010·Immunology and Cell Biology·Nusrat RahmanGraham Jones
Aug 14, 2013·PloS One·Sanna SiltanenJohanna Schleutker
Aug 6, 2014·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Youming ZhangMiriam F Moffatt
Aug 19, 2010·Pediatric Pulmonology·Hing Y SyTing F Leung
Jan 25, 2017·Genes & Development·Yi GongTitia de Lange
Aug 9, 2011·Current Opinion in Allergy and Clinical Immunology·Audrey PoonCatherine Laprise
Jun 30, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Emmanouil PapasavvasLuis J Montaner
Jul 28, 2021·Molecular Neurobiology·Shiek S S J AhmedRam Murugesan

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