Functional characterization of the copper transcription factor AfMac1 from Aspergillus fumigatus

The Biochemical Journal
Yong-Sung ParkCheol-Won Yun

Abstract

Although copper functions as a cofactor in many physiological processes, copper overload leads to harmful effects in living cells. Thus, copper homeostasis is tightly regulated. However, detailed copper metabolic pathways have not yet been identified in filamentous fungi. In this report, we investigated the copper transcription factor AfMac1 ( Aspergillus fumigatusMac1 homolog) and identified its regulatory mechanism in A. fumigatus AfMac1 has domains homologous to the DNA-binding and copper-binding domains of Mac1 from Saccharomyces cerevisiae, and AfMac1 efficiently complemented Mac1 in S. cerevisiae Expression of Afmac1 resulted in CTR1 up-regulation, and mutation of the DNA-binding domain of Afmac1 failed to activate CTR1 expression in S. cerevisiae The Afmac1 deletion strain of A. fumigatus failed to grow in copper-limited media, and its growth was restored by introducing ctrC We found that AfMac1 specifically bound to the promoter region of ctrC based on EMSA. The AfMac1-binding motif 5'-TGTGCTCA-3' was identified from the promoter region of ctrC, and the addition of mutant ctrC lacking the AfMac1-binding motif failed to up-regulate ctrC in A. fumigatus Furthermore, deletion of Afmac1 significantly reduced strain virulenc...Continue Reading

References

May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A DancisR D Klausner
Jun 14, 1996·The Journal of Biological Chemistry·D M GlerumA Tzagoloff
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·J A Graden, D R Winge
Sep 20, 1997·The Journal of Biological Chemistry·V C CulottaJ D Gitlin
May 23, 1998·Molecular and Cellular Biology·M M PeñaD J Thiele
Sep 3, 1998·The Journal of Biological Chemistry·L J MartinsD R Winge
Sep 10, 1999·The Journal of Biological Chemistry·C P Jamison McDanielsT D Tullius
Jun 5, 2003·Microbiology·Marcus E MarvinAnnette M Cashmore
Jun 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chang-Hoon WooJae-Hong Kim
Jan 1, 1953·Advances in Genetics·G PONTECORVOA W J BUFTON
Feb 10, 2007·The Journal of Clinical Investigation·Scott R WatermanPeter R Williamson
Oct 27, 2007·Biochemical and Biophysical Research Communications·Lucia BanciLeonardo Tenori
May 22, 2010·Molecular Microbiology·Karine LambouJean-Paul Latge
Apr 12, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Mark R Bleackley, Ross T A Macgillivray
Aug 16, 2011·Trends in Biochemical Sciences·Kate M Ehrensberger, Amanda J Bird
Oct 11, 2011·Microbial Cell Factories·Juan A Tamayo RamosLeo H de Graaff
Oct 11, 2011·Metallomics : Integrated Biometal Science·Christopher L DupontChristopher Rensing
Mar 6, 2012·The Journal of Biological Chemistry·Caroline C Philpott
Mar 7, 2013·Genetics·Martha S Cyert, Caroline C Philpott
Mar 19, 2013·Cell Host & Microbe·Chen DingDennis J Thiele
Apr 9, 2013·Revista Do Instituto De Medicina Tropical De São Paulo·Renan Marrichi MauchAmanda Latercia Tranches Dias
May 24, 2014·Molecular Microbiology·Chen DingZhan-You Wang
Jul 22, 2014·Current Medicinal Chemistry·H KozlowskiS Potocki
Oct 5, 2014·Fungal Genetics and Biology : FG & B·Yong-Sung ParkCheol-Won Yun
Jun 10, 2015·The Journal of Biological Chemistry·Sarela García-Santamarina, Dennis J Thiele
Feb 20, 2017·Current Genetics·Yoko KusuyaHiroki Takahashi

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Citations

Oct 1, 2018·The European Journal of Neuroscience·Yukihiro Yamada, Rebecca A Prosser
May 17, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Martzel Antsotegi-UskolaUnai Ugalde
Nov 15, 2019·Clinical Microbiology Reviews·Jean-Paul Latgé, Georgios Chamilos
Aug 20, 2020·The Biochemical Journal·Annie YapHubertus Haas
Aug 19, 2020·Molecular Microbiology·Edward M CulbertsonValeria C Culotta
Apr 30, 2019·Frontiers in Microbiology·Jinxing SongJihong Jiang
Dec 11, 2019·International Journal of Molecular Sciences·Hyewon SeoCheol-Won Yun
Apr 26, 2019·International Journal of Molecular Sciences·Nicholas RaffaNancy P Keller
Mar 3, 2020·Frontiers in Microbiology·Caitlin L WilliamsD Scott Merrell
Oct 22, 2020·International Journal of Molecular Sciences·Suzie KangCheol-Won Yun
Jan 12, 2021·Seminars in Cell & Developmental Biology·Edward M Culbertson, Valeria C Culotta
Feb 24, 2021·World Journal of Microbiology & Biotechnology·Vijaya RaniRajeev Kaushik
Apr 4, 2021·Journal of Fungi·Janelle R RobinsonFelicia N Anike

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