Functional characterization of the human biglycan 5'-flanking DNA and binding of the transcription factor c-Krox

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
A M HeegaardM Young

Abstract

The transcriptional regulation of human biglycan expression under normal and pathological conditions was studied. The 5'-flanking regions of the human and mouse genes were isolated and analyzed; the two promoter regions share 81% identity. Both promoters are without a TATA and CAT box and contain multiple Sp1 sites. Human dermal fibroblasts were transiently transfected with progressive deletional human biglycan 5'-flanking DNA-CAT constructs, and a significant variation in activity among the individual constructs was found. A small deletion in several cases caused a more than 2-fold increase or decrease in promoter activity, thereby mapping the target sites for repressors or activators. Human biglycan expression is reduced in females with Ullrich-Turner syndrome (45,X) and increased in individuals with supernumerary sex chromosomes, and it has been speculated that biglycan plays a role in the short stature phenotype of Turner syndrome. Analysis of the transcriptional regulation of biglycan in individuals with sex chromosome anomalies showed that a -262 to -218 region of the biglycan promoter was differentially regulated. This region was extensively analyzed by DNAse footprinting and electrophoretic mobility shift assays, and a ...Continue Reading

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Citations

Oct 8, 2004·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Bernhard Ganss, Andrew Jheon
Sep 17, 2004·Journal of Cellular Biochemistry·Anne-Marie HeegaardKarina Lishmann Nielsen
May 18, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·T GotoH Y Kubota
Feb 9, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·Boris SchmitzStefan-Martin Brand
Jul 20, 2007·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Vincenzo SollazzoFrancesco Carinci
Aug 19, 2007·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Vincenzo SollazzoFrancesco Carinci
Mar 20, 2010·Birth Defects Research. Part C, Embryo Today : Reviews·Xiao-Dong Chen
Dec 1, 2005·Journal of Cellular Biochemistry·Michael S FriedmanKurt D Hankenson
Apr 14, 2006·Cells, Tissues, Organs·Adele L BoskeyKostas Verdelis
Jul 26, 2002·The Journal of Biological Chemistry·Wen-Bin ChenHendrik Ungefroren
Jan 18, 2011·Molecular and Cellular Endocrinology·Gurvinder KenthCynthia Gates Goodyer

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