Functional characterization of the late embryogenesis abundant (LEA) protein gene family from Pinus tabuliformis (Pinaceae) in Escherichia coli

Scientific Reports
Jie Gao, Ting Lan

Abstract

Late embryogenesis abundant (LEA) proteins are a large and highly diverse gene family present in a wide range of plant species. LEAs are proposed to play a role in various stress tolerance responses. Our study represents the first-ever survey of LEA proteins and their encoding genes in a widely distributed pine (Pinus tabuliformis) in China. Twenty-three LEA genes were identified from the P. tabuliformis belonging to seven groups. Proteins with repeated motifs are an important feature specific to LEA groups. Ten of 23 pine LEA genes were selectively expressed in specific tissues, and showed expression divergence within each group. In addition, we selected 13 genes representing each group and introduced theses genes into Escherichia coli to assess the protective function of PtaLEA under heat and salt stresses. Compared with control cells, the E. coli cells expressing PtaLEA fusion protein exhibited enhanced salt and heat resistance and viability, indicating the protein may play a protective role in cells under stress conditions. Furthermore, among these enhanced tolerance genes, a certain extent of function divergence appeared within a gene group as well as between gene groups, suggesting potential functional diversity of this g...Continue Reading

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Citations

Jun 24, 2017·Cellular and Molecular Life Sciences : CMLS·Alejandra A CovarrubiasCaspar C C Chater
Jan 31, 2018·Nature Plants·Tao WanXiao-Ming Wang
Feb 15, 2019·Environmental Science and Pollution Research International·Tingzhang HuNong Zhou
Aug 11, 2020·The Plant Journal : for Cell and Molecular Biology·Qiuyue MaQianzhong Li
Jan 16, 2020·International Journal of Molecular Sciences·Sara HamzelouPaul A Haynes
Mar 13, 2020·Scientific Reports·Marimuthu KumaravelMarimuthu Somasundaram Saraswathi
Nov 6, 2018·International Journal of Molecular Sciences·Blaise Pascal MuvunyiJiyu Zhang
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Jul 25, 2019·Scientific Reports·Jose Miguel Soriano, Fanny Alvaro
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Jan 2, 2020·Evolutionary Applications·Amanda R De La TorreIlga Porth
Apr 23, 2020·International Journal of Molecular Sciences·Patrick Knox-BrownAnja Thalhammer
Jun 3, 2018·Biotechnology Letters·Nadil ShahChunyu Zhang
Jan 12, 2021·Journal of Plant Physiology·Chunmei HeJun Duan

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Methods Mentioned

BETA
transgenic
environmental stresses
PCR
electrophoresis

Software Mentioned

BioEdit
targetP
Cellprofiler
MEGA
Multiple Expectation maximization for Motif Elucidation ( MEME )
MUSCLE
MEME
TBLASTN

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