Functional characterization of the PP2C phosphatase CaPtc2p in the human fungal pathogen Candida albicans

Yeast
Jinrong FengLinghuo Jiang

Abstract

Type 2C protein phosphatases (PP2C) are monomeric enzymes and their activities require the presence of magnesium or manganese ion. There are seven PP2C-like genes in Candida albicans. In this study, we demonstrate that CaPtc2p is a PP2C phosphatase. Surprisingly, in addition to the cytoplasmic localization, CaPtc2p is partially associated with mitochondria in yeast-form and filamentous cells of C. albicans. Expression of CaPTC2 is developmentally regulated during the serum-induced filamentation. Deletion of CaPTC2 renders C. albicans cells sensitive to SDS and azole antifungals, as well as the DNA methylation agent methylmethane sulphonate and the DNA synthesis inhibitor hydroxyurea. Therefore, CaPtc2p might fulfil multiple functions, including the regulation of mitochondrial physiology and checkpoint recovery from DNA damage in C. albicans cells.

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Citations

Nov 16, 2010·Eukaryotic Cell·Joaquín AriñoAsier González
Dec 27, 2015·Medical Mycology·Mohammad T Albataineh, David Kadosh
Jan 20, 2015·Molecular Microbiology·Lizziane K WinkelströterGustavo H Goldman
Jul 9, 2013·Protoplasma·Alejandro Tovar-MendezDouglas D Randall
May 7, 2015·G3 : Genes - Genomes - Genetics·Lizziane K WinkelströterGustavo H Goldman
Dec 6, 2019·Molecular Plant Pathology·Juan Carlos Nunez-RodriguezConcepcion Hera

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