Functional characterization of the putative Aspergillus nidulans poly(ADP-ribose) polymerase homolog PrpA

Genetics
Camile P SemighiniSteven D Harris

Abstract

Poly(ADP-ribose) polymerase (PARP) is a highly conserved enzyme involved in multiple aspects of animal and plant cell physiology. For example, PARP is thought to be intimately involved in the early signaling events that trigger the DNA damage response. However, the genetic dissection of PARP function has been hindered by the presence of multiple homologs in most animal and plant species. Here, we present the first functional characterization of a putative PARP homolog (PrpA) in a microbial system (Aspergillus nidulans). PrpA belongs to a group of PARP homologs that includes representatives from filamentous fungi and protists. The genetic analysis of prpA demonstrates that it is an essential gene whose role in the DNA damage response is sensitive to gene dosage. Notably, temporal patterns of prpA expression and PrpA-GFP nuclear localization suggest that PrpA acts early in the A. nidulans DNA damage response. Additional studies implicate PrpA in farnesol-induced cell death and in the initiation of asexual development. Collectively, our results provide a gateway for probing the diverse functions of PARP in a sophisticated microbial genetic system.

References

Jun 1, 1988·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·G de MurciaG G Poirier
Jan 1, 1973·The Journal of Heredity·E Käfer, A Upshall
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G G PoirierP Mandel
Aug 1, 1997·Fungal Genetics and Biology : FG & B·S D Harris
Apr 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·T H AdamsJ H Yu
Aug 24, 1999·The Biochemical Journal·D D'AmoursG G Poirier
Apr 11, 2001·Mutation Research·G J Hageman, R H Stierum
Jul 20, 2001·Experimental Cell Research·L DavidovicG G Poirier
Feb 21, 2002·The Journal of Clinical Investigation·J G Seidman, Christine Seidman
Apr 4, 2002·Fungal Genetics and Biology : FG & B·Gustavo H GoldmanSteven D Harris
Sep 21, 2002·Science·Kathleen Heppner GossJoanna Groden
Dec 17, 2002·Molecular and Cellular Biology·Jijun ChengXiang S Ye
Jan 16, 2003·Trends in Microbiology·Hervé Lalucque, Philippe Silar
May 3, 2003·The EMBO Journal·Josiane Ménissier de MurciaGilbert de Murcia
May 16, 2003·Molecular and Cellular Biology·Satoshi OkanoAkira Yasui
Jun 7, 2003·Molecular Microbiology·Camile P SemighiniGustavo Henrique Goldman
Jun 28, 2003·Experimental Hematology·Véronique J BouchardGuy G Poirier
Jul 11, 2003·Nature·Balázs PappLaurence D Hurst
May 25, 2004·Molecular Biology of the Cell·Claire L PearsonSteven D Harris
May 27, 2004·Cytogenetic and Genome Research·M P Hande
Jun 5, 2004·Biochemical and Biophysical Research Communications·Fumiaki WatanabeYoshimasa Uehara
Jul 27, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jean-Christophe AméGilbert de Murcia
Oct 27, 2004·Trends in Biochemical Sciences·J N Mark GloverMegan S Lee
Nov 9, 2004·Current Opinion in Cell Biology·Aimee L Edinger, Craig B Thompson
Feb 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Changbin Chen, Martin B Dickman
Feb 18, 2005·Genetics·Adam M DeutschbauerGuri Giaever
May 3, 2005·Nature Reviews. Drug Discovery·Prakash Jagtap, Csaba Szabó

❮ Previous
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Citations

Oct 13, 2006·Eukaryotic Cell·Iran MalavaziGustavo Henrique Goldman
Dec 7, 2010·Eukaryotic Cell·Bárbara de Castro Pimentel FigueiredoGustavo Henrique Goldman
Jul 16, 2013·Biochemistry. Biokhimii︠a︡·G A ShilovskyS I Shram
Mar 10, 2011·BMC Developmental Biology·Jyotika RajawatRasheedunnisa Begum
Oct 15, 2010·BMC Evolutionary Biology·Matteo CitarelliRebecca S Lamb
Jan 25, 2012·Medical Mycology·Patrícia Albuquerque, Arturo Casadevall
Aug 24, 2011·Cellular and Molecular Life Sciences : CMLS·Rebecca S LambSachin Teotia
Jan 5, 2010·Fungal Genetics and Biology : FG & B·Gregory O KotheHirokazu Inoue
Apr 29, 2008·Trends in Microbiology·Andrea HamannHeinz D Osiewacz
May 29, 2013·The FEBS Journal·Eva BarkauskaiteGyula Timinszky
Mar 30, 2016·Differentiation; Research in Biological Diversity·Tina JubinRasheedunnisa Begum
Oct 13, 2006·Current Opinion in Microbiology·N Louise Glass, Karine Dementhon
Nov 17, 2012·Microbiology·Melinda SzilágyiTamás Emri
May 7, 2009·FEMS Microbiology Reviews·Amir SharonIdo Hatam
Aug 2, 2016·Seminars in Cell & Developmental Biology·Tina JubinRasheedunnisa Begum
Nov 12, 2016·Fungal Genetics and Biology : FG & B·Margaret E KatzJoan M Kelly
Mar 29, 2018·Nature Microbiology·Neil Andrew BrownGustavo Henrique Goldman
May 6, 2019·Cellular and Molecular Life Sciences : CMLS·Yueshuang KeXueqing Ba
Oct 7, 2017·Frontiers in Microbiology·A Pedro GonçalvesN Louise Glass
Oct 27, 2018·Expert Opinion on Therapeutic Targets·Éva LeiterIstván Pócsi

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