Functional Characterization of the Tau Class Glutathione-S-Transferases Gene (SbGSTU) Promoter of Salicornia brachiata under Salinity and Osmotic Stress

PloS One
Vivekanand TiwariBhavanath Jha

Abstract

Reactive oxygen or nitrogen species are generated in the plant cell during the extreme stress condition, which produces toxic compounds after reacting with the organic molecules. The glutathione-S-transferase (GST) enzymes play a significant role to detoxify these toxins and help in excretion or sequestration of them. In the present study, we have cloned 1023 bp long promoter region of tau class GST from an extreme halophyte Salicornia brachiata and functionally characterized using the transgenic approach in tobacco. Computational analysis revealed the presence of abiotic stress responsive cis-elements like ABRE, MYB, MYC, GATA, GT1 etc., phytohormones, pathogen and wound responsive motifs. Three 5'-deletion constructs of 730 (GP2), 509 (GP3) and 348 bp (GP4) were made from 1023 (GP1) promoter fragment and used for tobacco transformation. The single event transgenic plants showed notable GUS reporter protein expression in the leaf tissues of control as well as treated plants. The expression level of the GUS gradually decreases from GP1 to GP4 in leaf tissues, whereas the highest level of expression was detected with the GP2 construct in root and stem under control condition. The GUS expression was found higher in leaves and ste...Continue Reading

References

Feb 21, 1998·Nucleic Acids Research·K HigoH Higo
Dec 17, 2003·The Plant Journal : for Cell and Molecular Biology·Satoshi KitamuraAtsushi Tanaka
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·Kathleen A. Marrs
Feb 24, 2006·Current Opinion in Biotechnology·Taishi UmezawaKazuo Shinozaki
Jul 15, 2006·Biomolecular Engineering·Carla Frova
Jun 21, 2008·The New Phytologist·Timothy J Flowers, Timothy D Colmer
Feb 26, 2009·Antioxidants & Redox Signaling·Christine H Foyer, Graham Noctor
Aug 29, 2009·Plant, Cell & Environment·Gad MillerRon Mittler
Feb 9, 2010·Plant Physiology and Biochemistry : PPB·Geert PottersMarcel A K Jansen
Sep 28, 2010·Plant Physiology and Biochemistry : PPB·Sarvajeet Singh Gill, Narendra Tuteja
Jan 1, 2010·The Arabidopsis Book·David P Dixon, Robert Edwards
Aug 12, 2014·Current Opinion in Plant Biology·Takuya YoshidaKazuko Yamaguchi-Shinozaki

❮ Previous
Next ❯

Citations

Apr 10, 2017·Plant Cell Reports·Irini Nianiou-ObeidatNikolaos E Labrou
Jan 11, 2018·International Journal of Molecular Sciences·Gai-Li NiuMuhammad Ashraf
Jun 14, 2019·Frontiers in Plant Science·Elodie Sylvestre-GononArnaud Hecker
Mar 11, 2020·Planta·Shabir Hussain WaniP B Kavi Kishor
Jul 14, 2020·3 Biotech·Swati VaishMahesh Kumar Basantani
Jan 29, 2019·Frontiers in Plant Science·Ágnes GalléPéter Poór
Jun 3, 2017·Frontiers in Plant Science·Avinash Mishra, Bhakti Tanna
Mar 11, 2020·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Sonam Yadav, Avinash Mishra
Jul 6, 2017·International Journal of Molecular Sciences·Agnieszka BielachVanesa B Tognetti

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
electrophoresis
PCR
X-ray
Fluorescence
Protein Assay

Software Mentioned

PlantCARE
PLACE

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.