Functional characterization of three (GH13) branching enzymes involved in cyanobacterial starch biosynthesis from Cyanobacterium sp. NBRC 102756

Biochimica Et Biophysica Acta
Ryuichiro SuzukiEiji Suzuki

Abstract

Starch and glycogen are widespread storage polysaccharides in bacteria, plants, and animals. Recently, some cyanobacteria were found to accumulate water-insoluble α-glucan similar to amylopectin rather than glycogen, the latter of which is more commonly produced in these organisms. The amylopectin-producing species including Cyanobacterium sp. NBRC 102756 invariably have three branching enzyme (BE) homologs, BE1, BE2, and BE3, all belonging to the glycoside hydrolase family 13. Multiple BE isoforms in prokaryotes have not been previously studied. In the present work, we carried out functional characterization of these enzymes expressed in Escherichia coli. The recombinant enzymes were all active, although the specific activity of BE3 was much lower than those of BE1 and BE2. After the incubation of the enzymes with amylopectin or amylose, the reaction products were analyzed by fluorophore-assisted carbohydrate capillary electrophoresis method. BE1 and BE2 showed similar chain-length preference to BEIIb isoform of rice (Oryza sativa L.), while the catalytic specificity of BE3 was similar to that of rice BEI. These results indicate that starch-producing cyanobacteria have both type-I BE (BE3) and type-II BEs (BE1 and BE2) in term...Continue Reading

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Citations

May 5, 2016·Cellular and Molecular Life Sciences : CMLS·Eiji Suzuki, Ryuichiro Suzuki
Feb 15, 2017·The Journal of Biological Chemistry·Mari HayashiEiji Suzuki
Jan 31, 2020·Critical Reviews in Biotechnology·Xiaofeng BanZhaofeng Li
Nov 30, 2018·Frontiers in Plant Science·Casper WilkensMarie Sofie Møller
Aug 8, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Mari HayashiEiji Suzuki
Mar 5, 2021·Archives of Biochemistry and Biophysics·Yousra El MannaiEiji Suzuki
Jun 26, 2021·International Journal of Biological Macromolecules·Chengyao XiaZhongli Cui
Mar 3, 2018·Methods : a Companion to Methods in Enzymology·Srikanth GattuLisa A Holland

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