Abstract
THY1 was previously identified as a candidate tumor suppressor gene (TSG) associated with lymph node metastases in nasopharyngeal carcinoma (NPC) through functional studies. It was identified by oligonucleotide microarray analysis as an interesting differentially expressed gene. However, direct functional evidence is still lacking for THY1 being a TSG in NPC, as in vivo tumorigenicity assays have not been previously reported in our last study of THY1. In this study, a tetracycline-inducible expression vector, pETE-Bsd, was used to obtain stable transfectants of THY1. The stringent in vivo tumorigenicity assay results show that the activation of THY1 suppresses tumor formation of HONE1 cells in nude mice, and the tumor formation ability was restored in the presence of doxycycline (a tetracycline analog), when the gene is shut off. Functional inactivation of this gene is observed in all the tumors derived from the tumorigenic transfectant. The tumor suppressive effect could be repressed by knockdown of THY1 expression in nontumorigenic microcell hybrids. Further studies indicate that expression of THY1 inhibits HONE1 cell growth in vitro by arresting cells in G(0)/G(1) phase. It greatly reduces the ability for anchorage-independe...Continue Reading
References
Jan 1, 1997·British Journal of Cancer·A MutiranguraN Voravud
May 25, 1999·Genes, Chromosomes & Cancer·Y J ChenC H Lin
May 26, 1999·Experimental Hematology·M Barda-SaadD Zipori
Mar 30, 2000·Genes, Chromosomes & Cancer·Y ChengM L Lung
Jun 21, 2002·Microvascular Research·A SaalbachU Anderegg
Jul 19, 2002·The Journal of Gene Medicine·Alexei I ProtopopovEugene R Zabarovsky
May 15, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Angela Kwok Fung LoGeorge Sai Wah Tsao
Jun 5, 2003·Cancer Genetics and Cytogenetics·Harindra R AbeysingheNancy Wang
Mar 31, 2004·Proceedings of the National Academy of Sciences of the United States of America·Vladimir I KashubaEugene R Zabarovsky
Apr 20, 2004·Experimental Cell Research·Thomas H BarkerJames S Hagood
Apr 24, 2004·Cancer Genetics and Cytogenetics·Harindra R AbeysingheNancy Wang
Jun 23, 2004·Oncogene·Jingfeng LiEugene Zabarovsky
Sep 18, 2004·Cancer Research·Jingfeng LiEugene R Zabarovsky
Sep 24, 2004·International Journal of Cancer. Journal International Du Cancer·Hong Lok LungMaria Li Lung
Jul 12, 2005·Oncogene·Hong Lok LungMaria Li Lung
Aug 17, 2005·Cancer Genetics and Cytogenetics·Harindra R AbeysingheNancy Wang
May 12, 2006·International Journal of Cancer. Journal International Du Cancer·H M LiS W Tsao
Sep 26, 2006·Biochimica Et Biophysica Acta·Tanya A Rege, James S Hagood
Oct 5, 2006·Cancer Research·Hong Lok LungMaria Li Lung
Oct 19, 2007·International Journal of Cancer. Journal International Du Cancer·Alfred Chi Chung LeungMaria Li Lung
Oct 24, 2007·Pediatric Surgery International·Henning C FiegelDietrich Kluth
Nov 22, 2007·International Journal of Cancer. Journal International Du Cancer·Hong Lok LungMaria Li Lung
Feb 5, 2008·Cancer Cell·Zhen Fan YangSheung Tat Fan
Feb 16, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Zhen Fan YangSheung Tat Fan
Oct 3, 2008·Cancer Research·Arthur Kwok Leung CheungMaria Li Lung
Citations
Jun 17, 2010·Cancer Research·Paulisally Hau Yi LoMaria Li Lung
Dec 6, 2011·PloS One·Zhen LiuWeiyi Fang
Nov 7, 2013·PloS One·Ida JohanssonIngrid Hedenfalk
Dec 14, 2011·Seminars in Cancer Biology·Hong Lok LungMaria Li Lung
Dec 14, 2011·Cancer Letters·Paulisally Hau Yi LoMaria Li Lung
Jan 29, 2011·Acta histochemica·Jeng-Wei LuRouh-Mei Hu
Aug 25, 2015·Gastroenterology·Valen Zhuoyou YuMaria Li Lung
May 9, 2016·Pathology, Research and Practice·Lile HeZhihong Li
May 15, 2015·PloS One·Rebecca KanMaria Li Lung
Jul 29, 2016·Stem Cell Research & Therapy·Daniela A MoraesDaniela M Oliveira
May 20, 2014·American Journal of Clinical Pathology·Giosuè ScognamiglioRenato Franco
Oct 15, 2019·International Journal of Cancer. Journal International Du Cancer·Merrin M L LeongMaria L Lung
Oct 30, 2016·Oncotarget·Annie Wai Yeeng ChaiMaria Li Lung
Jul 28, 2020·Cancers·Mingdan DengMaria Li Lung
Mar 16, 2021·Frontiers in Oncology·Merrin Man Long Leong, Maria Li Lung