Functional characterization of THY1 as a tumor suppressor gene with antiinvasive activity in nasopharyngeal carcinoma

International Journal of Cancer. Journal International Du Cancer
Hong Lok LungMaria L Lung

Abstract

THY1 was previously identified as a candidate tumor suppressor gene (TSG) associated with lymph node metastases in nasopharyngeal carcinoma (NPC) through functional studies. It was identified by oligonucleotide microarray analysis as an interesting differentially expressed gene. However, direct functional evidence is still lacking for THY1 being a TSG in NPC, as in vivo tumorigenicity assays have not been previously reported in our last study of THY1. In this study, a tetracycline-inducible expression vector, pETE-Bsd, was used to obtain stable transfectants of THY1. The stringent in vivo tumorigenicity assay results show that the activation of THY1 suppresses tumor formation of HONE1 cells in nude mice, and the tumor formation ability was restored in the presence of doxycycline (a tetracycline analog), when the gene is shut off. Functional inactivation of this gene is observed in all the tumors derived from the tumorigenic transfectant. The tumor suppressive effect could be repressed by knockdown of THY1 expression in nontumorigenic microcell hybrids. Further studies indicate that expression of THY1 inhibits HONE1 cell growth in vitro by arresting cells in G(0)/G(1) phase. It greatly reduces the ability for anchorage-independe...Continue Reading

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Citations

Dec 14, 2011·Seminars in Cancer Biology·Hong Lok LungMaria Li Lung
Dec 14, 2011·Cancer Letters·Paulisally Hau Yi LoMaria Li Lung
May 9, 2016·Pathology, Research and Practice·Lile HeZhihong Li
Jul 29, 2016·Stem Cell Research & Therapy·Daniela A MoraesDaniela M Oliveira
May 20, 2014·American Journal of Clinical Pathology·Giosuè ScognamiglioRenato Franco
Oct 15, 2019·International Journal of Cancer. Journal International Du Cancer·Merrin M L LeongMaria L Lung

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