Functional consequences of amino acid substitutions to GerVB, a component of the Bacillus megaterium spore germinant receptor.

Journal of Bacteriology
Graham ChristieChristopher R Lowe

Abstract

The extreme metabolic dormancy and resistance properties of spores formed by members of the Bacillus and Clostridium genera are lost upon exposure to a variety of small-molecule germinants. Germinants are known to interact in an as yet undefined manner with cognate receptor complexes that reside in the inner membrane that surrounds the spore protoplast. The receptor itself is a complex of at least three proteins, and in this study we identify amino acid residues, predicted to lie in loop regions of GerVB on the exterior aspect of the membrane, that influence the Bacillus megaterium spore germination response. Three consecutive residues adjacent to putative transmembrane domain 10 (TM10) were demonstrated to mediate to various degrees the proline germinative response while also influencing germination in response to leucine, glucose, and inorganic salts, suggesting that this region may be part of a ligand binding pocket. Alternatively, substitutions in this region may affect the conformation of associated functionally important TM regions. Leucine- and KBr-mediated germination was also influenced by substitutions in other outer loop regions. These observations, when considered with accompanying kinetic analyses that demonstrate ...Continue Reading

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Citations

Feb 3, 2011·Current Microbiology·Anastasia PapalazaridouAfroditi Sivropoulou
Apr 30, 2013·Journal of Bacteriology·Arturo Ramirez-PeraltaPeter Setlow
Apr 30, 2013·Journal of Bacteriology·Srishti GuptaGraham Christie
Apr 24, 2014·BMC Microbiology·Elisabeth H MadslienToril Lindbäck
Nov 30, 2010·Trends in Microbiology·Daniel Paredes-SabjaMahfuzur R Sarker
Jul 27, 2010·Journal of Molecular Biology·Yunfeng LiBing Hao
Nov 15, 2016·Frontiers in Microbiology·Jason BruntMichael W Peck
May 23, 2019·Proceedings of the National Academy of Sciences of the United States of America·Yunfeng LiBing Hao
Jun 24, 2016·Microbiology Spectrum·Anne Moir, Gareth Cooper

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