Functional conservation of the transportin nuclear import pathway in divergent organisms.

Molecular and Cellular Biology
M C SiomiG Dreyfuss

Abstract

Human transportin1 (hTRN1) is the nuclear import receptor for a group of pre-mRNA/mRNA-binding proteins (heterogeneous nuclear ribonucleoproteins [hnRNP]) represented by hnRNP A1, which shuttle continuously between the nucleus and the cytoplasm. hTRN1 interacts with the M9 region of hnRNP A1, a 38-amino-acid domain rich in Gly, Ser, and Asn, and mediates the nuclear import of M9-bearing proteins in vitro. Saccharomyces cerevisiae transportin (yTRN; also known as YBR017c or Kap104p) has been identified and cloned. To understanding the nuclear import mediated by yTRN, we searched with a yeast two-hybrid system for proteins that interact with it. In an exhaustive screen of the S. cerevisiae genome, the most frequently selected open reading frame was the nuclear mRNA-binding protein, Nab2p. We delineated a ca.-50-amino-acid region in Nab2p, termed NAB35, which specifically binds yTRN and is similar to the M9 motif. NAB35 also interacts with hTRN1 and functions as a nuclear localization signal in mammalian cells. Interestingly, yTRN can also mediate the import of NAB35-bearing proteins into mammalian nuclei in vitro. We also report on additional substrates for TRN as well as sequences of Drosophila melanogaster, Xenopus laevis, and ...Continue Reading

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Citations

Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·X C Fan, J A Steitz
Nov 17, 1998·The Journal of Cell Biology·J S RosenblumG Blobel
Jun 8, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D A JansM H Lam
Sep 1, 2000·FEBS Letters·P Legrain, L Selig
Apr 16, 2009·The Journal of Biological Chemistry·Katherine E Süel, Yuh Min Chook
May 2, 2014·FEBS Letters·Laure TwyffelsVéronique Kruys
Oct 8, 2009·Human Molecular Genetics·G Scott PesiridisJohn Q Trojanowski
Jul 31, 2009·Molecular Biology of the Cell·Corine K LauDouglass J Forbes
Aug 10, 2000·Archives of Biochemistry and Biophysics·Y AraoS Kato
Jun 6, 2002·Drug Discovery Today·Vincent Schächter
Oct 30, 2010·Biochimica Et Biophysica Acta·Yuh Min Chook, Katherine E Süel
Mar 3, 2006·Genome Research·Sourav BandyopadhyayTrey Ideker

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