Functional consortia for cresol-degrading activated sludges: toxicity-to-extinction approach

Bioresource Technology
Kuo-Ling HoDuu-Jong Lee

Abstract

The conventional roll tube and plating techniques are typically time consuming and can culture in vitro only a small fraction of microbes in natural microflora. This study utilizes a novel, simple, and rapid method, the toxicity-to-extinction approach, to obtain the minimal functional consortium that can effectively degrade meta- (m-), para- (p-), and ortho- (o-) cresols. The original sludge had 16 major bands by denaturing gradient gel electrophoresis (DGGE). Microbial diversity decreased as the cresol concentration increased. The functional strains acquired under toxic stress by dosed cresols that individually degraded m-, p-, and o-cresols were identified. Catechol 1,2-dioxygenase (C12D) and catechol 2,3-dioxygenase (C23D) activities in cell-free extracts were determined spectrophotometrically and were correlated with noted changes in microbial communities under cresol stress. The proposed toxicity-to-extinction approach is feasible for isolating a functional consortium from sludge for cresol degradation.

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Citations

Jun 27, 2012·Applied Microbiology and Biotechnology·H MikeskováK Svobodová
Dec 18, 2012·Environmental Technology·F Basheer, I H Farooqi
Sep 7, 2014·Journal of Environmental Sciences (China)·Carlos David SilvaAnne-Claire Texier
Feb 20, 2016·Environmental Science and Pollution Research International·Juan Antonio Salas-CortésAnne-Claire Texier
Aug 27, 2011·Bioresource Technology·C S SrinandanAnuradha S Nerurkar
Feb 28, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Nuria Fernandez-GonzalezJose Luis Sanz

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