Functional coordination of non-myocytes plays a key role in adult zebrafish heart regeneration.

EMBO Reports
Hong MaJiandong Liu


Cardiac regeneration occurs primarily through proliferation of existing cardiomyocytes, but also involves complex interactions between distinct cardiac cell types including non-cardiomyocytes (non-CMs). However, the subpopulations, distinguishing molecular features, cellular functions, and intercellular interactions of non-CMs in heart regeneration remain largely unexplored. Using the LIGER algorithm, we assemble an atlas of cell states from 61,977 individual non-CM scRNA-seq profiles isolated at multiple time points during regeneration. This analysis reveals extensive non-CM cell diversity, including multiple macrophage (MC), fibroblast (FB), and endothelial cell (EC) subpopulations with unique spatiotemporal distributions, and suggests an important role for MC in inducing the activated FB and EC subpopulations. Indeed, pharmacological perturbation of MC function compromises the induction of the unique FB and EC subpopulations. Furthermore, we developed computational algorithm Topologizer to map the topological relationships and dynamic transitions between functional states. We uncover dynamic transitions between MC functional states and identify factors involved in mRNA processing and transcriptional regulation associated wit...Continue Reading


Feb 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·K KajiA Kudo
Aug 9, 2002·Developmental Biology·Nathan D Lawson, Brant M Weinstein
Aug 27, 2002·Current Biology : CB·Hinrich HabeckUNKNOWN Tübingen 2000 screen consortium
Dec 14, 2002·Science·Kenneth D PossMark T Keating
Jun 7, 2003·The Journal of Infectious Diseases·Alan Aderem
Aug 12, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jon D LarsonJeffrey J Essner
Jul 16, 2005·Blood·Julien Y BertrandIsabelle Godin
Aug 16, 2005·Blood·Hui-Feng LinRobert I Handin
Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
Feb 6, 2007·Developmental Cell·Hongtao Yu
May 31, 2008·Contributions to Microbiology·Chahrazade KantariVéronique Witko-Sarsat
Sep 2, 2008·Microvascular Research·Rebecca G BagleyBeverly A Teicher
Oct 22, 2008·Experimental Cell Research·Maaike S Pols, Judith Klumperman
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Mar 21, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Cynthia D CooperDavid W Raible
Jun 23, 2009·Mechanisms of Development·Massimo M SantoroDidier Y Stainier
Nov 7, 2009·Circulation Research·Paige SniderSimon J Conway
Mar 26, 2010·Nature·Chris JoplingJuan Carlos Izpisúa Belmonte
Jul 2, 2010·Blood·Kevin B WaltersAnna Huttenlocher
Nov 19, 2010·Blood·Felix EllettGraham J Lieschke
Feb 26, 2011·Science·Enzo R PorrelloHesham A Sadek
Mar 17, 2011·Blood·Valerie WittamerDavid Traver
May 20, 2011·Nature·Michael A Laflamme, Charles E Murry
Oct 19, 2011·Development·Aniket V GoreBrant M Weinstein
Mar 28, 2012·Current Topics in Developmental Biology·Paul R Riley
Jan 22, 2013·Nature Neuroscience·Katrin KierdorfMarco Prinz
Mar 30, 2013·Nature Protocols·Veronika SanderJuan Carlos Izpisua Belmonte
Apr 19, 2013·Nature·Ahmed I MahmoudHesham A Sadek
Apr 19, 2013·Nature·Ross N W KettleboroughDerek L Stemple
Feb 14, 2014·Development·Andreas van ImpelStefan Schulte-Merker
Feb 27, 2014·The Journal of Clinical Investigation·Arin B AuroraEric N Olson
May 9, 2014·Nature·Jop H van BerloJeffery D Molkentin

❮ Previous
Next ❯

Related Concepts

Related Feeds

CZI Human Cell Atlas Seed Network

The aim of the Human Cell Atlas (HCA) is to build reference maps of all human cells in order to enhance our understanding of health and disease. The Seed Networks for the HCA project aims to bring together collaborators with different areas of expertise in order to facilitate the development of the HCA. Find the latest research from members of the HCA Seed Networks here.